| http://purl.uniprot.org/citations/23087210 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23087210 | http://www.w3.org/2000/01/rdf-schema#comment | "Synaptosomal associated protein of 23 kDa (SNAP-23), a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE), has been implicated in phagocytosis by macrophages. For elucidation of its precise role in this process, a macrophage line overexpressing monomeric Venus-tagged SNAP-23 was established. These cells showed enhanced Fc receptor-mediated phagocytosis. Detailed analyses of each process of phagocytosis revealed a marked increase in the production of reactive oxygen species within phagosomes. Also, enhanced accumulation of a lysotropic dye, as well as augmented quenching of a pH-sensitive fluorophore were observed. Analyses of isolated phagosomes indicated the critical role of SNAP-23 in the functional recruitment of the NADPH oxidase complex and vacuolar-type H(+)-ATPase to phagosomes. The data from the overexpression experiments were confirmed by SNAP-23 knockdown, which demonstrated a significant delay in phagosome maturation and a reduction in uptake activity. Finally, for analyzing whether phagosomal SNAP-23 entails a structural change in the protein, an intramolecular Förster resonance energy transfer (FRET) probe was constructed, in which the distance within a TagGFP2-TagRFP was altered upon close approximation of the N-termini of its two SNARE motifs. FRET efficiency on phagosomes was markedly enhanced only when VAMP7, a lysosomal SNARE, was coexpressed. Taken together, our results strongly suggest the involvement of SNAP-23 in both phagosome formation and maturation in macrophages, presumably by mediating SNARE-based membrane traffic."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e12-01-0069"xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Arai S."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Hashimoto H."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Wada Y."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Nakanishi H."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Hatsuzawa K."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Wada I."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Sun-Wada G.H."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/author | "Sakurai C."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/pages | "4849-4863"xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/title | "SNAP-23 regulates phagosome formation and maturation in macrophages."xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/23087210 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23087210 |
| http://purl.uniprot.org/citations/23087210 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23087210 |
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| http://purl.uniprot.org/uniprot/#_A2AKH4-mappedCitation-23087210 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23087210 |
| http://purl.uniprot.org/uniprot/#_B0R029-mappedCitation-23087210 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23087210 |
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| http://purl.uniprot.org/uniprot/#_E9Q8A1-mappedCitation-23087210 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23087210 |
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| http://purl.uniprot.org/uniprot/#_O00161-mappedCitation-23087210 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23087210 |