| http://purl.uniprot.org/citations/23091054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23091054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23091054 | http://www.w3.org/2000/01/rdf-schema#comment | "Post-transcriptional modifications of the wobble uridine (U34) of tRNAs play a critical role in reading NNA/G codons belonging to split codon boxes. In a subset of Escherichia coli tRNA, this wobble uridine is modified to 5-methylaminomethyluridine (mnm(5)U34) through sequential enzymatic reactions. Uridine 34 is first converted to 5-carboxymethylaminomethyluridine (cmnm(5)U34) by the MnmE-MnmG enzyme complex. The cmnm(5)U34 is further modified to mnm(5)U by the bifunctional MnmC protein. In the first reaction, the FAD-dependent oxidase domain (MnmC1) converts cmnm(5)U into 5-aminomethyluridine (nm(5)U34), and this reaction is immediately followed by the methylation of the free amino group into mnm(5)U34 by the S-adenosylmethionine-dependent domain (MnmC2). Aquifex aeolicus lacks a bifunctional MnmC protein fusion and instead encodes the Rossmann-fold protein DUF752, which is homologous to the methyltransferase MnmC2 domain of Escherichia coli MnmC (26% identity). Here, we determined the crystal structure of the A. aeolicus DUF752 protein at 2.5 Å resolution, which revealed that it catalyzes the S-adenosylmethionine-dependent methylation of nm(5)U in vitro, to form mnm(5)U34 in tRNA. We also showed that naturally occurring tRNA from A. aeolicus contains the 5-mnm group attached to the C5 atom of U34. Taken together, these results support the recent proposal of an alternative MnmC1-independent shortcut pathway for producing mnm(5)U34 in tRNAs."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M112.409300"xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112.409300"xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Bessho Y."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Bessho Y."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Shibata R."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Shibata R."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Grosjean H."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Grosjean H."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Sengoku T."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Sengoku T."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Nishimoto M."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Nishimoto M."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Kitamura A."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Kitamura A."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Bjork G.R."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Bjork G.R."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Jager G."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/author | "Jager G."xsd:string |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23091054 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |