http://purl.uniprot.org/citations/23095890 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23095890 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23095890 | http://www.w3.org/2000/01/rdf-schema#comment | "Left-right (L-R) patterning is essential for proper organ morphogenesis and function. Calcium fluxes in dorsal forerunner cells (DFCs) are known to regulate the formation of Kupffer's vesicle (KV), a central organ for establishing L-R asymmetry in zebrafish. Here, we identify the lipid mediator lysophosphatidic acid (LPA) as a regulator of L-R asymmetry in zebrafish embryos. LPA is produced by Autotaxin (Atx), a secreted lysophospholipase D, and triggers various cellular responses through activation of specific G protein-coupled receptors (Lpar1-6). Knockdown of Atx or LPA receptor 3 (Lpar3) by morpholino oligonucleotides perturbed asymmetric gene expression in lateral plate mesoderm and disrupted organ L-R asymmetries, whereas overexpression of lpar3 partially rescued those defects in both atx and lpar3 morphants. Similar defects were observed in embryos treated with the Atx inhibitor HA130 and the Lpar1-3 inhibitor Ki16425. Knockdown of either Atx or Lpar3 impaired calcium fluxes in DFCs during mid-epiboly stage and compromised DFC cohesive migration, KV formation and ciliogenesis. Application of LPA to DFCs rescued the calcium signal and laterality defects in atx morphants. This LPA-dependent L-R asymmetry is mediated via Wnt signaling, as shown by the accumulation of β-catenin in nuclei at the dorsal side of both atx and lpar3 morphants. Our results suggest a major role for the Atx/Lpar3 signaling axis in regulating KV formation, ciliogenesis and L-R asymmetry via a Wnt-dependent pathway."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.org/dc/terms/identifier | "doi:10.1242/dev.081745"xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Moolenaar W.H."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Moolenaar W.H."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Ovaa H."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Ovaa H."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Tsao K.C."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Tsao K.C."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Albers H.M."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Albers H.M."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Houben A.J."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Houben A.J."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Yao W.L."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Yao W.L."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Lai S.L."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/author | "Lai S.L."xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/name | "Development"xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/name | "Development"xsd:string |
http://purl.uniprot.org/citations/23095890 | http://purl.uniprot.org/core/pages | "4439-4448"xsd:string |