| http://purl.uniprot.org/citations/23115245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23115245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23115245 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein kinase A-anchoring proteins (AKAPs) provide spatio-temporal specificity for the omnipotent cAMP-dependent protein kinase (PKA) via high affinity interactions with PKA regulatory subunits (PKA-RI, RII). Many PKA-RII-AKAP complexes are heavily tethered to cellular substructures, whereas PKA-RI-AKAP complexes have remained largely undiscovered. Here, using a cAMP affinity-based chemical proteomics strategy in human heart and platelets, we uncovered a novel, ubiquitously expressed AKAP, termed small membrane (sm)AKAP due to its specific localization at the plasma membrane via potential myristoylation/palmitoylation anchors. In vitro binding studies revealed specificity of smAKAP for PKA-RI (K(d) = 7 nM) over PKA-RII (K(d) = 53 nM) subunits, co-expression of smAKAP with the four PKA R subunits revealed an even more exclusive specificity of smAKAP for PKA-RIα/β in the cellular context. Applying the singlet oxygen-generating electron microscopy probe miniSOG indicated that smAKAP is tethered to the plasma membrane and is particularly dense at cell-cell junctions and within filopodia. Our preliminary functional characterization of smAKAP provides evidence that, like PKA-RII, PKA-RI can be tightly tethered by a novel repertoire of AKAPs, providing a new perspective on spatio-temporal control of cAMP signaling."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112.395970"xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m112.395970"xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Heck A.J."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Heck A.J."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Ma Y."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Ma Y."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Taylor S.S."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Taylor S.S."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "van der Heyden M.A."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "van der Heyden M.A."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Scholten A."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Scholten A."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Mackey M."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Mackey M."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Burgers P.P."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Burgers P.P."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Ellisman M."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Ellisman M."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Margarucci L."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/author | "Margarucci L."xsd:string |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23115245 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |