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http://purl.uniprot.org/citations/23137016http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23137016http://www.w3.org/2000/01/rdf-schema#comment"

Background

Infectious agents, especially Streptococcus sanguinis and herpes simplex virus, have long been postulated as major triggering factors for Behçet disease (BD).

Objectives

To identify an anti-S. sanguinis antigen reacting with serum IgA antibody in patients with BD.

Methods

We detected a target protein by proteomics analysis and evaluated serum IgA reactivity of 100 patients with BD against the identified streptococcal target protein and human heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1. Homologous epitope sequences between the streptococcal target protein and human hnRNP A2/B1 were also evaluated.

Results

Four protein bands were detected by immunoprecipitation, and chaperonin GroEL was identified by a proteomics analysis. Reactivity of serum IgA against recombinant S. sanguinis GroEL was detected in 77 of 100 patients with BD (77%) and in 21 of 70 healthy controls (30%). In addition, reactivity of serum IgA against human recombinant hnRNP A2/B1 was seen in 79 of 100 patients with BD (79%) and in eight of 70 healthy controls (11%). Among the eight distinctive epitopes with significant homology between S. sanguinis GroEL and human hnRNP A2/B1, the serum IgA reactivity of patients with BD was markedly higher with epitope 3 (hnRNP A2/B1 peptide 33-46 and GroEL peptide 57-70) and epitope 6 (hnRNP A2/B1 peptide 177-188 and GroEL peptide 347-358).

Conclusion

We identified an S. sanguinis GroEL protein as a target of serum anti-S. sanguinis IgA antibody reactivity in patients with BD. In addition, patients with BD exhibited serum IgA reactivity against homologous epitope regions between S. sanguinis GroEL and human hnRNP A2/B1."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.org/dc/terms/identifier"doi:10.1111/bjd.12128"xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Kim D.Y."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Zheng Z."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Lee H.S."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Choi M.J."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Cho S."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Cho S.B."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Ahn K.J."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/author"Bang D."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/name"Br J Dermatol"xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/pages"977-983"xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/title"Serum IgA reactivity against GroEL of Streptococcus sanguinis and human heterogeneous nuclear ribonucleoprotein A2/B1 in patients with Behcet disease."xsd:string
http://purl.uniprot.org/citations/23137016http://purl.uniprot.org/core/volume"168"xsd:string
http://purl.uniprot.org/citations/23137016http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23137016
http://purl.uniprot.org/citations/23137016http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/23137016
http://purl.uniprot.org/uniprot/#_P22626-mappedCitation-23137016http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23137016
http://purl.uniprot.org/uniprot/P22626http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23137016