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http://purl.uniprot.org/citations/23159077http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23159077http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To observe the expression of SFRP1 gene methylation in non-small cell lung cancer (NSCLC), and study the effect of 5-Aza-2-deoxycytidine (5-Aza-CdR) on DNA methylation and expression of SFRP1, p16 and MGMT genes in the human lung cancer cell line SPC-A-1 cells.

Methods

SP immunohistochemistry and methylation-specific PCR were used to detect the SFRP1 methylation in 60 NSCLC cases, and 21 cases of benign lung diseases were used as control group. SPC-A-1 cells were cultured and treated with 5-Aza-CdR. The promoter methylation status of SFRP1, p16 and MGMT genes were detected by methylation-specific polymerase (MSP) chain reaction, and mRNAs were detected by real-time PCR.

Results

The positive rate of SFRP1 gene methylation in NSCLC was significantly higher than that in normal lung tissue (58.3% vs. 14.3%; χ(2) = 12.118, P = 0.001). SFRP1 gene methylation was closely correlated with lymph node metastasis and degree of differentiation in NSCLC (P < 0.05). SFRP1 protein expression was correlated with clinical stage, degree of differentiation and lymph node metastasis in NSCLC (P < 0.05). The positive expression of SFRP1 protein in 30 cases of NSCLC tissue containing SFRP1 gene methylation was significantly higher than that in non-methylated NSCLC (68.6% vs. 24.0%; χ(2) = 9.613, P = 0.002). SFRP1 gene methylation was closely correlated with SFRP1 gene protein expression in NSCLC (P < 0.05). Negative expression of SFRP1 protein was correlated with the differentiation, clinical stage, and lymph node metastasis in NSCLC (all P < 0.05). Without 5-Aza-CdR treatment, the expressions of methylation of SFRP1, p16 and MGMT genes and their mRNA were low. After 5-Aza-CdR treatment at different concentrations, their expressions were significantly elevated (all P < 0.05).

Conclusions

SFRP1 gene methylation is closely associated with carcinogenesis and development of NSCLC. 5-Aza-CdR may reverse the methylation of SFRP1, p16 and MGMT genes, and facilitate the re-expression of the anti-oncogenes."xsd:string
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http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/author"Zhu H.B."xsd:string
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/author"Yu Z.C."xsd:string
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/author"Jin Y.T."xsd:string
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/author"Fang H.L."xsd:string
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/name"Zhonghua Zhong Liu Za Zhi"xsd:string
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/pages"658-663"xsd:string
http://purl.uniprot.org/citations/23159077http://purl.uniprot.org/core/title"[Effects of 5-Aza-2-deoxycytidine on DNA methylation of anti-oncogenes in non-small cell lung cancer cells]."xsd:string
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