| http://purl.uniprot.org/citations/2318885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2318885 | http://www.w3.org/2000/01/rdf-schema#comment | "Cloned 15-lipoxygenase has been expressed for the first time in eukaryotic and prokaryotic cells. Transfection of osteosarcoma cells with a mammalian expression plasmid containing the cDNA for human reticulocyte 15-lipoxygenase resulted in cell lines that were capable of oxidizing body arachidonic acid and linoleic acid. The lipoxygenase metabolites were identified by reverse-phase and straight-phase high pressure liquid chromatography, ultraviolet spectroscopy, and direct mass spectrometry, verifying that the cDNA for 15-lipoxygenase encodes an enzyme with authentic 15-lipoxygenase activity. Incubation of the transformed cells with arachidonic acid generated 15-hydroxyeicosatetraenoic acid (HETE) and 12-HETE in a ratio of 8.6 to 1, demonstrating that 15-lipoxygenase can also perform 12-lipoxygenation. Lesser amounts of 15-keto-ETE, four isomers of 8,15-diHETE, and one isomer of 14,15-diHETE were observed. Incubation with linoleic acid generated predominantly 13-hydroxy linoleic acid. The reaction was inhibited by eicosatetraynoic acid but not by indomethacin. Antibodies to a peptide corresponding to a unique region of the predicted amino acid sequence were generated and shown to react with one major band of 70 kDa on immunoblots of human leukocyte 15-lipoxygenase. To obtain antibodies to the full length enzyme, the cDNA was subcloned into a bacterial expression vector and was expressed as a fusion with the CheY protein. The overexpressed protein was readily purified from bacteria and was shown to be immunoreactive to the peptide-derived antibody. Antibodies raised to this recombinant enzyme did not cross-react with human leukocyte 5-lipoxygenase but did identify 15-lipoxygenase in rabbit reticulocytes, human leukocytes, and tracheal epithelial cells, suggesting that the 15-lipoxygenases from these different cell types are structurally related."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)34092-x"xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/author | "Dixon R.A."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/author | "Craik C.S."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/author | "Gross C."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/author | "Grunberger D."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/author | "Sigal E."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/author | "Highland E."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/pages | "5113-5120"xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/title | "Expression of cloned human reticulocyte 15-lipoxygenase and immunological evidence that 15-lipoxygenases of different cell types are related."xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/2318885 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2318885 |
| http://purl.uniprot.org/citations/2318885 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2318885 |
| http://purl.uniprot.org/uniprot/#_P18054-mappedCitation-2318885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2318885 |
| http://purl.uniprot.org/uniprot/#_P16050-mappedCitation-2318885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2318885 |
| http://purl.uniprot.org/uniprot/P16050 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/2318885 |
| http://purl.uniprot.org/uniprot/P18054 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/2318885 |