Results

RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/23196258http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23196258http://www.w3.org/2000/01/rdf-schema#comment"

Background

The induction of specific immune tolerance for alloantigen is the best method for solving transplant rejection. We have previously reported T-cell tolerance induced by RNA interference (RNAi) RelB dendritic cells (DCs), supporting the possibility of immunologic tolerance in liver transplantation.

Methods

A stable model of acute rejection was established in Lewis (RT11) rats that had received a liver graft from dark agouti-RT1a rats. To evaluate the immune tolerance of DCs of different maturity, the rats were randomly assigned to four groups (12 donor/recipient pairs): (1) control-DC group, recipient rats without preinjection; (2) RelB short hairpin (sh)RNAi-DC group, recipient rats with preinjection of tolerogenic DCs by way of RelB silencing; (3) imDC group, recipient rats with preinjection of immature DCs; and (4) lipopolysaccharide-DC group, recipient rats with preinjection of mature DCs. The immune tolerance of the grafts was evaluated by liver function tests (aspartate transaminase, total bilirubin), cytokines (interleukin [IL]-2, IL-4, IL-10 and interferon-γ), and histopathologic examination during the 2 wk after transplantation. The survival time of the rats was also observed.

Results

Compared with the other three groups, the graft survival time was significantly prolonged in the RelB shRNAi-DC group. In addition, RelB shRNAi-DCs resulted in the reduced secretion of IL-2 and interferon-γ and increased levels of IL-10 and IL-4. The symptoms of rejection were obviously alleviated in the RelB shRNAi-DC group, and the rejection activity index was still reduced after 2 wk.

Conclusions

Injection of RelB-silenced DCs contributed to the reduced incidence of graft rejection and prolonged the graft survival time. The potential mechanisms involved the regulation and induction of immune-incompetent T cell by DCs."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.org/dc/terms/identifier"doi:10.1016/j.jss.2012.10.021"xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Bao J."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Dong R."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Ma Y."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Tang Z."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Xie J."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Wen H."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/author"Zou Z."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/name"J Surg Res"xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/pages"169-175"xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/title"Immune tolerance induced by RelB short-hairpin RNA interference dendritic cells in liver transplantation."xsd:string
http://purl.uniprot.org/citations/23196258http://purl.uniprot.org/core/volume"180"xsd:string
http://purl.uniprot.org/citations/23196258http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23196258
http://purl.uniprot.org/citations/23196258http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/23196258
http://purl.uniprot.org/uniprot/#_D3Z9V1-mappedCitation-23196258http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23196258
http://purl.uniprot.org/uniprot/#_D4A8H1-mappedCitation-23196258http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23196258
http://purl.uniprot.org/uniprot/D3Z9V1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23196258
http://purl.uniprot.org/uniprot/D4A8H1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23196258