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http://purl.uniprot.org/citations/23211777http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23211777http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Lens epithelium-derived growth factor (LEDGF)/p75 is a cellular binding partner of HIV-1 integrase and a crucial cofactor for HIV-1 replication. Here, we study two LEDGF/p75 exonic variants I436S and T473I, identified in HIV-1 long-term nonprogressors, together with Q472L.

Methods

In-vitro binding assays, cell culture complementation, and functional rescue.

Results

Binding affinities of wild-type, I436S, T473I, and Q472L LEDGF/p75 for HIV-1 integrase were comparable. All LEDGF/p75 variants bound equally well to LEDGF/p75 interacting partners JPO2 and PogZ. In addition, HIV-1 replication was evaluated in human somatic LEDGF/p75-knockout cells and LEDGF/p75-knockdown cells complemented with either wild-type LEDGF/p75 or the respective LEDGF/p75 variants. All variants rescued HIV-1 replication to wild-type levels, whereas LEDGF/p75 D366N, defective for interaction with HIV-1 integrase, did not.

Conclusion

Although identified in a cohort of long-term nonprogressors, our study did not indicate that the I436S or T473I mutation in LEDGF/p75 affects the interaction with HIV-1 integrase."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.org/dc/terms/identifier"doi:10.1097/qad.0b013e32835d0d86"xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"Gerard M."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"Christ F."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"De Rijck J."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"Debyser Z."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"Gijsbers R."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"Demeulemeester J."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/author"Schrijvers R."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/name"AIDS"xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/pages"539-543"xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/title"Characterization of rare lens epithelium-derived growth factor/p75 genetic variants identified in HIV-1 long-term nonprogressors."xsd:string
http://purl.uniprot.org/citations/23211777http://purl.uniprot.org/core/volume"27"xsd:string
http://purl.uniprot.org/citations/23211777http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23211777
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http://purl.uniprot.org/uniprot/Q05CM9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23211777
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