| http://purl.uniprot.org/citations/23212245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23212245 | http://www.w3.org/2000/01/rdf-schema#comment | "Construction and intracellular targeting of eukaryotic pre-ribosomal particles involve a multitude of diverse transiently associating trans-acting assembly factors, energy-consuming enzymes, and transport factors. The ability to rapidly and reliably measure co-enrichment of multiple factors with maturing pre-ribosomal particles presents a major biochemical bottleneck towards revealing their function and the precise contribution of >50 energy-consuming steps that drive ribosome assembly. Here, we devised a workflow that combines genetic trapping, affinity-capture, and selected reaction monitoring mass spectrometry (SRM-MS), to overcome this deficiency. We exploited this approach to interrogate the dynamic proteome of pre-60S particles after nuclear export. We uncovered assembly factors that travel with pre-60S particles to the cytoplasm, where they are released before initiating translation. Notably, we identified a novel shuttling factor that facilitates nuclear export of pre-60S particles. Capturing and quantitating protein interaction networks of trapped intermediates of macromolecular complexes by our workflow is a reliable discovery tool to unveil dynamic processes that contribute to their in vivo assembly and transport."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.org/dc/terms/identifier | "doi:10.1038/msb.2012.63"xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Chang Y."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Picotti P."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Schutz S."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Panse V.G."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Melnik A."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Occhipinti L."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Rothenbusch U."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/author | "Altvater M."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/name | "Mol Syst Biol"xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/pages | "628"xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/title | "Targeted proteomics reveals compositional dynamics of 60S pre-ribosomes after nuclear export."xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/23212245 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23212245 |
| http://purl.uniprot.org/citations/23212245 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23212245 |
| http://purl.uniprot.org/uniprot/Q08004#attribution-70EBFFAA7AA9AE1517B9614ED8C7AAC6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23212245 |
| http://purl.uniprot.org/uniprot/Q08004#attribution-85007C01AA286E870E05109390914C8D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23212245 |
| http://purl.uniprot.org/uniprot/P0C2H7#attribution-2A1DFC6B5C713B28BF26612ED003991C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23212245 |
| http://purl.uniprot.org/uniprot/P0C2H7#attribution-85007C01AA286E870E05109390914C8D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23212245 |
| http://purl.uniprot.org/uniprot/#_P0C2H7-mappedCitation-23212245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23212245 |
| http://purl.uniprot.org/uniprot/#_P14126-mappedCitation-23212245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23212245 |
| http://purl.uniprot.org/uniprot/#_P53863-mappedCitation-23212245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23212245 |