| http://purl.uniprot.org/citations/23233545 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23233545 | http://www.w3.org/2000/01/rdf-schema#comment | "Transcriptional enhancement of X-linked genes to compensate for the sex chromosome monosomy in Drosophila males is brought about by a ribonucleoprotein assembly called Male-Specific-Lethal or Dosage Compensation Complex (MSL-DCC). This machinery is formed in male flies and specifically associates with active genes on the X chromosome. After assembly at dedicated high-affinity "entry" sites (HAS) on the X chromosome, the complex distributes to the nearby active chromatin. High-resolution, genome-wide mapping of the MSL-DCC subunits by chromatin immunoprecipitation (ChIP) on oligonucleotide tiling arrays suggests a rather homogenous spreading of the intact complex onto transcribed chromatin. Coupling ChIP to deep sequencing (ChIP-seq) promises to map the chromosomal interactions of the DCC with improved resolution. We present ChIP-seq binding profiles for all complex subunits, including the first description of the RNA helicase MLE binding pattern. Exploiting the preferential representation of direct chromatin contacts upon high-energy shearing, we report a surprising functional and topological separation of MSL protein contacts at three classes of chromosomal binding sites. Furthermore, precise determination of DNA fragment lengths by paired-end ChIP-seq allows decrypting of the local complex architecture. Primary contacts of MSL-2 and MLE define HAS for the DCC. In contrast, association of the DCC with actively transcribed gene bodies is mediated by MSL-3 binding to nucleosomes. We identify robust MSL-1/MOF binding at a fraction of active promoters genome-wide. Correlation analyses suggest that this association reflects a function outside dosage compensation. Our comprehensive analysis provides a new level of information on different interaction modes of a multiprotein complex at distinct regions within the genome."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.146407.112"xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/author | "Becker P.B."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/author | "Straub T."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/author | "Gilfillan G.D."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/author | "Feller C."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/author | "Zabel A."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/name | "Genome Res"xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/pages | "473-485"xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/title | "Different chromatin interfaces of the Drosophila dosage compensation complex revealed by high-shear ChIP-seq."xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/23233545 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23233545 |
| http://purl.uniprot.org/citations/23233545 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23233545 |
| http://purl.uniprot.org/uniprot/#_P50536-mappedCitation-23233545 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23233545 |
| http://purl.uniprot.org/uniprot/#_P24785-mappedCitation-23233545 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23233545 |
| http://purl.uniprot.org/uniprot/P50536 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23233545 |
| http://purl.uniprot.org/uniprot/P24785 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23233545 |