| http://purl.uniprot.org/citations/23233731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23233731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23233731 | http://www.w3.org/2000/01/rdf-schema#comment | "The mycelium of the rice stem pathogen, Magnaporthe salvinii, secreted linoleate 9S-lipoxygenase (9S-LOX) and epoxy alcohol synthase (EAS). The EAS rapidly transformed 9S-hydroperoxy-octadeca-10E,12Z-dienoic acid (9S-HPODE) to threo 10 (11)-epoxy-9S-hydroxy-12Z-octadecenoic acid, but other hydroperoxy FAs were poor substrates. 9S-LOX was expressed in Pichia pastoris. Recombinant 9S-LOX oxidized 18:2n-6 directly to 9S-HPODE, the end product, and also to two intermediates, 11S-hydroperoxy-9Z,12Z-octadecenoic acid (11S-HPODE; ∼5%) and 13R-hydroperoxy-9Z,11E-octadecadienoic acid (13R-HPODE; ∼1%). 11S- and 13R-HPODE were isomerized to 9S-HPODE, probably after oxidation to peroxyl radicals, β-fragmentation, and oxygen insertion at C-9. The 18:3n-3 was oxidized at C-9, C-11, and C-13, and to 9,16-dihydroxy-10E,12,14E-octadecatrienoic acid. 9S-LOX contained catalytic manganese (Mn:protein ∼0.2:1; Mn/Fe, 1:0.05), and its sequence could be aligned with 77% identity to 13R-LOX with catalytic manganese lipoxygenase (13R-MnLOX) of the Take-all fungus. The Leu350Met mutant of 9S-LOX shifted oxidation of 18:2n-6 from C-9 to C-13, and the Phe347Leu, Phe347Val, and Phe347Ala mutants of 13R-MnLOX from C-13 to C-9. In conclusion, M. salvinii secretes 9S-LOX with catalytic manganese along with a specific EAS. Alterations in the Sloane determinant of 9S-LOX and 13R-MnLOX with larger and smaller hydrophobic residues interconverted the regiospecific oxidation of 18:2n-6, presumably by altering the substrate position in relation to oxygen insertion."xsd:string |
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| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/author | "Oliw E.H."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/author | "Oliw E.H."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/author | "Wennman A."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/author | "Wennman A."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/name | "J. Lipid Res."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/name | "J. Lipid Res."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/pages | "762-775"xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/pages | "762-775"xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/title | "Secretion of two novel enzymes, manganese 9S-lipoxygenase and epoxy alcohol synthase, by the rice pathogen Magnaporthe salvinii."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/title | "Secretion of two novel enzymes, manganese 9S-lipoxygenase and epoxy alcohol synthase, by the rice pathogen Magnaporthe salvinii."xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/volume | "54"xsd:string |
| http://purl.uniprot.org/citations/23233731 | http://purl.uniprot.org/core/volume | "54"xsd:string |
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| http://purl.uniprot.org/citations/23233731 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23233731 |
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| http://purl.uniprot.org/citations/23233731 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23233731 |
| http://purl.uniprot.org/uniprot/Q8X151 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/23233731 |
| http://purl.uniprot.org/uniprot/P0CT92 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/23233731 |