| http://purl.uniprot.org/citations/23234360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23234360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23234360 | http://www.w3.org/2000/01/rdf-schema#comment | "The GalNAc O-glycosylation on Ser/Thr residues of extracellular proteins has not been well characterized from a proteomics perspective. We previously reported a sialic acid capture-and-release protocol to enrich tryptic N- and O-glycopeptides from human cerebrospinal fluid glycoproteins using nano-LC-ESI-MS/MS with collision-induced dissociation (CID) for glycopeptide characterization. Here, we have introduced peptide N-glycosidase F (PNGase F) pretreatment of CSF samples to remove the N-glycans facilitating the selective characterization of O-glycopeptides and enabling the use of an automated CID-MS(2)/MS(3) search protocol for glycopeptide identification. We used electron-capture and -transfer dissociation (ECD/ETD) to pinpoint the glycosylation site(s) of the glycopeptides, identified as predominantly core-1-like HexHexNAc-O-structure attached to one to four Ser/Thr residues. We characterized 106 O-glycosylations and found Pro residues preferentially in the n - 1, n + 1, and/or n + 3 positions in relation to the Ser/Thr attachment site (n). The characterization of glycans and glycosylation sites in glycoproteins from human clinical samples provides a basis for future studies addressing the biological and diagnostic importance of specific protein glycosylations in relation to human disease."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.org/dc/terms/identifier | "doi:10.1021/pr300963h"xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.org/dc/terms/identifier | "doi:10.1021/pr300963h"xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Nilsson J."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Nilsson J."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Halim A."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Halim A."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Larson G."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Larson G."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Ruetschi U."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/author | "Ruetschi U."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/name | "J. Proteome Res."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/name | "J. Proteome Res."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/pages | "573-584"xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/pages | "573-584"xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/title | "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/title | "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/23234360 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23234360 |
| http://purl.uniprot.org/citations/23234360 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23234360 |