| http://purl.uniprot.org/citations/23242998 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23242998 | http://www.w3.org/2000/01/rdf-schema#comment | "Yeast cells polarize by projecting up mating pheromone gradients, a classic cell polarity behavior. However, these chemical gradients may shift direction. We examine how yeast cells sense and respond to a 180(o) switch in the direction of microfluidically generated pheromone gradients. We identify two behaviors: at low concentrations of α-factor, the initial projection grows by bending, whereas at high concentrations, cells form a second projection toward the new source. Mutations that increase heterotrimeric G-protein activity expand the bending-growth morphology to high concentrations; mutations that increase Cdc42 activity result in second projections at low concentrations. Gradient-sensing projection bending requires interaction between Gβγ and Cdc24, whereas gradient-nonsensing projection extension is stimulated by Bem1 and hyperactivated Cdc42. Of interest, a mutation in Gα affects both bending and extension. Finally, we find a genetic perturbation that exhibits both behaviors. Overexpression of the formin Bni1, a component of the polarisome, makes both bending-growth projections and second projections at low and high α-factor concentrations, suggesting a role for Bni1 downstream of the heterotrimeric G-protein and Cdc42 during gradient sensing and response. Thus we demonstrate that G-proteins modulate in a ligand-dependent manner two fundamental cell-polarity behaviors in response to gradient directional change."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e12-10-0739"xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/author | "Kim H.J."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/author | "Tanaka H."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/author | "Jeon N.L."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/author | "Moore T.I."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/author | "Yi T.M."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/pages | "521-534"xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/title | "Yeast G-proteins mediate directional sensing and polarization behaviors in response to changes in pheromone gradient direction."xsd:string |
| http://purl.uniprot.org/citations/23242998 | http://purl.uniprot.org/core/volume | "24"xsd:string |
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