| http://purl.uniprot.org/citations/23271279 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23271279 | http://www.w3.org/2000/01/rdf-schema#comment | "Recent studies showed that activation of Wnt/β-catenin pathway promoted the differentiation of osteoblast-like cells in the arterial calcification, but its mechanism remains unknown. In this study, the hypothesis that Wnt/β-catenin pathway promotes the differentiation of osteoblast-like cells by upregulating the expression of receptor activator of NF-κB ligand (RANKL) was examined. LiCl was used to activate the Wnt/β-catenin pathway. The differentiation of osteoblast-like cells was observed by Von Kossa staining, calcium content assay, alkaline phosphatase (ALP) activity assay, and detection of osteocalcin expression. Real-time PCR was performed to detect the expression of RANKL and osteoprotegerin (OPG, the decoy receptor of RANKL) during the osteoblast-like cell differentiation. Different concentrations of OPG were added to the culture media respectively to inhibit the function of RANKL, and the change in the differentiation of osteoblast-like cells was evaluated. The results showed that when the Wnt/β-catenin pathway was activated by LiCl, the expression of RANKL was significantly increased, which coincided with the differentiation of osteoblast-like cells (P<0.05), and the OPG treatment could partly attenuate the promoting effect of Wnt/β-catenin pathway on the differentiation of osteoblast-like cells (P<0.05), but it failed to completely abolish such effect. It was concluded that activation of Wnt/β-catenin pathway promotes the differentiation of osteoblast-like cells by both RANKL-dependent and RANKL-independent mechanisms."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11596-012-1040-4"xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/author | "Wang B."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/author | "Zhou S."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/author | "Fang X."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/author | "Guan S."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/author | "Nie B."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/name | "J Huazhong Univ Sci Technolog Med Sci"xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/pages | "818-822"xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/title | "Implication of receptor activator of NF-kappaB ligand in Wnt/beta-catenin pathway promoting osteoblast-like cell differentiation."xsd:string |
| http://purl.uniprot.org/citations/23271279 | http://purl.uniprot.org/core/volume | "32"xsd:string |
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