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http://purl.uniprot.org/citations/23275563http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23275563http://www.w3.org/2000/01/rdf-schema#comment"The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens were performed for the human neurodegenerative disease proteins huntingtin and ataxin-1. Using systematic controls for unspecific Y2H results and quantitative benchmarking, we identified and scored a large number of known and novel partner proteins for both huntingtin and ataxin-1. Moreover, we show that this parallelized screening procedure and the global inspection of Y2H interaction data are uniquely suited to define specific PPI patterns and their alteration by disease-causing mutations in huntingtin and ataxin-1. This approach takes advantage of the specificity and flexibility of DNA microarrays and of the existence of solid-related statistical methods for the analysis of DNA microarray data, and allows a quantitative approach toward interaction screens in human and in model organisms."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.org/dc/terms/identifier"doi:10.1093/nar/gks1329"xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Herwig R."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Andrade-Navarro M.A."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Suter B."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Wanker E.E."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Porras P."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Saar K."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Rasko T."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Zenkner M."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Rau K."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Rasche A."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Russ J."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Yildirimman R."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Fontaine J.F."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Foulle R."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Schaefer M.H."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/author"Vazquez-Alvarez B.M."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/name"Nucleic Acids Res"xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/pages"1496-1507"xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/title"Development and application of a DNA microarray-based yeast two-hybrid system."xsd:string
http://purl.uniprot.org/citations/23275563http://purl.uniprot.org/core/volume"41"xsd:string
http://purl.uniprot.org/citations/23275563http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23275563
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