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| http://purl.uniprot.org/citations/23292935 | http://www.w3.org/2000/01/rdf-schema#comment | "Actin filament nucleation by actin-related protein (Arp) 2/3 complex is a critical process in cell motility and endocytosis, yet key aspects of its mechanism are unknown due to a lack of real-time observations of Arp2/3 complex through the nucleation process. Triggered by the verprolin homology, central, and acidic (VCA) region of proteins in the Wiskott-Aldrich syndrome protein (WASp) family, Arp2/3 complex produces new (daughter) filaments as branches from the sides of preexisting (mother) filaments. We visualized individual fluorescently labeled Arp2/3 complexes dynamically interacting with and producing branches on growing actin filaments in vitro. Branch formation was strikingly inefficient, even in the presence of VCA: only ~1% of filament-bound Arp2/3 complexes yielded a daughter filament. VCA acted at multiple steps, increasing both the association rate of Arp2/3 complexes with mother filament and the fraction of filament-bound complexes that nucleated a daughter. The results lead to a quantitative kinetic mechanism for branched actin assembly, revealing the steps that can be stimulated by additional cellular factors."xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1211164110"xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/author | "Goode B.L."xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/author | "Smith B.A."xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/author | "Gelles J."xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/author | "Daugherty-Clarke K."xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/pages | "1285-1290"xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/title | "Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging."xsd:string |
| http://purl.uniprot.org/citations/23292935 | http://purl.uniprot.org/core/volume | "110"xsd:string |
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