| http://purl.uniprot.org/citations/23298764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23298764 | http://www.w3.org/2000/01/rdf-schema#comment | "Migration of vascular smooth muscle cells (VSMCs) is essential for repair of vascular injury, development of atherosclerotic lesions and restenosis after angioplasty or by-pass graft surgery. It has been reported that platelet-derived growth factor (PDGF)-BB induces VSMC migration via the p44/p42 mitogen-activated protein (MAP) kinase pathway and the phosphatidylinositol 3 (PI3)-kinase/Akt pathway. Adenosine monophosphate-activated protein kinase (AMPK) is generally known to regulate multiple metabolic pathway. In the present study, we investigated the involvement of AMPK in PDGF-BB-induced migration of VSMCs using, a VSMC line, A10 cells. PDGF-BB induced phosphorylation of AMPK-α at Thr-172 residue. Treatment of A10 cells with compound C, an AMPK inhibitor, suppressed PDGF-BB-induced migration in a concentration-dependent manner (0.01-1μM). Compound C truly attenuated PDGF-BB induced phosphorylation of acetyl-CoA carboxylase, a downstream substance of AMPK. Downregulation of AMPK-α expression by the siRNA appeared an anti-migratory effect on PDGF-BB-induced migration. PDGF-BB-induced phosphorylation of c-Raf, MEK1/2 or p44/p42 MAP kinase, and phosphorylation of PI3-kinase or Akt were markedly suppressed by compound C. In conclusion, our results strongly suggest that PDGF-BB induces activation of AMPK in VSMCs, and subsequently regulates the migration via both the p44/p42 MAP kinase pathway and the PI3-kinase/Akt pathway."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.abb.2012.12.019"xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/author | "Tanabe K."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/author | "Iida H."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/author | "Iida M."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/author | "Kozawa O."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/author | "Matsushima-Nishiwaki R."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/name | "Arch Biochem Biophys"xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/pages | "83-92"xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/title | "Adenosine monophosphate-activated protein kinase regulates platelet-derived growth factor-BB-induced vascular smooth muscle cell migration."xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://purl.uniprot.org/core/volume | "530"xsd:string |
| http://purl.uniprot.org/citations/23298764 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23298764 |
| http://purl.uniprot.org/citations/23298764 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23298764 |
| http://purl.uniprot.org/uniprot/#_A0A221LG85-mappedCitation-23298764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/#_Q63740-mappedCitation-23298764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/#_P54646-mappedCitation-23298764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/#_Q05028-mappedCitation-23298764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/P54646 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/Q63740 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/A0A221LG85 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23298764 |
| http://purl.uniprot.org/uniprot/Q05028 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23298764 |