| http://purl.uniprot.org/citations/23319637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23319637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23319637 | http://www.w3.org/2000/01/rdf-schema#comment | "Bioactive gibberellins (GAs) control many aspects of growth and development in plants. GA(1) has been the most frequently found bioactive GA in various tissues of flowering plants, but the enzymes responsible for GA(1) biosynthesis have not been fully elucidated due to the enzymes catalyzing the 13-hydroxylation step not being identified. Because of the lack of mutants defective in this enzyme, biological significance of GA 13-hydroxylation has been unknown. Here, we report that two cytochrome P450 genes, CYP714B1 and CYP714B2, encode GA 13-oxidase in rice. Transgenic Arabidopsis plants that overexpress CYP714B1 or CYP714B2 show semidwarfism. There was a trend that the levels of 13-OH GAs including GA(1) were increased in these transgenic plants. Functional analysis using yeast or insect cells shows that recombinant CYP714B1 and CYP714B2 proteins can convert GA(12) into GA(53) (13-OH GA(12)) in vitro. Moreover, the levels of 13-OH GAs including GA(1) were decreased, whereas those of 13-H GAs including GA(4) (which is more active than GA(1)) were increased, in the rice cyp714b1 cyp714b2 double mutant. These results indicate that CYP714B1 and CYP714B2 play a predominant role in GA 13-hydroxylation in rice. The double mutant plants appear phenotypically normal until heading, but show elongated uppermost internode at the heading stage. Moreover, CYP714B1 and CYP714B2 expression was up-regulated by exogenous application of bioactive GAs. Our results suggest that GA 13-oxidases play a role in fine-tuning plant growth by decreasing GA bioactivity in rice and that they also participate in GA homeostasis."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1215788110"xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1215788110"xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Kawaide H."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Kawaide H."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Kamiya Y."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Kamiya Y."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Yamaguchi S."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Yamaguchi S."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Nomura T."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Nomura T."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Ohnishi T."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Ohnishi T."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Hanada A."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Hanada A."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Katsumata T."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Katsumata T."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Magome H."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Magome H."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Shinma Y."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Shinma Y."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Takeda-Kamiya N."xsd:string |
| http://purl.uniprot.org/citations/23319637 | http://purl.uniprot.org/core/author | "Takeda-Kamiya N."xsd:string |