| http://purl.uniprot.org/citations/23325844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23325844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23325844 | http://www.w3.org/2000/01/rdf-schema#comment | "Histone H3 of nucleosomes positioned on active genes is trimethylated at Lys36 (H3K36me3) by the SETD2 (also termed KMT3A/SET2 or HYPB) methyltransferase. Previous studies in yeast indicated that H3K36me3 prevents spurious intragenic transcription initiation through recruitment of a histone deacetylase complex, a mechanism that is not conserved in mammals. Here, we report that downregulation of SETD2 in human cells leads to intragenic transcription initiation in at least 11% of active genes. Reduction of SETD2 prevents normal loading of the FACT (FAcilitates Chromatin Transcription) complex subunits SPT16 and SSRP1, and decreases nucleosome occupancy in active genes. Moreover, co-immunoprecipitation experiments suggest that SPT16 is recruited to active chromatin templates, which contain H3K36me3-modified nucleosomes. Our results further show that within minutes after transcriptional activation, there is a SETD2-dependent reduction in gene body occupancy of histone H2B, but not of histone H3, suggesting that SETD2 coordinates FACT-mediated exchange of histone H2B during transcription-coupled nucleosome displacement. After inhibition of transcription, we observe a SETD2-dependent recruitment of FACT and increased histone H2B occupancy. These data suggest that SETD2 activity modulates FACT recruitment and nucleosome dynamics, thereby repressing cryptic transcription initiation."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gks1472"xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gks1472"xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Carmo-Fonseca M."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Carmo-Fonseca M."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Carvalho S."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Carvalho S."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Grosso A.R."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Grosso A.R."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Sridhara S.C."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Sridhara S.C."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "de Almeida S.F."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "de Almeida S.F."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Martins F.B."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Martins F.B."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Raposo A.C."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Raposo A.C."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Rino J."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/author | "Rino J."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/23325844 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |