| http://purl.uniprot.org/citations/23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23349838 | http://www.w3.org/2000/01/rdf-schema#comment | "Type 2 diabetes mellitus (T2DM) describes a group of metabolic disorders characterized by defects in insulin secretion and insulin sensitivity. Insulin secretion from pancreatic β-cells is an important factor in the etiology of T2DM, though the complex regulation and mechanisms of insulin secretion from β-cells remains to be fully elucidated. High plasma levels of serotonin (5-hydroxytryptamine; 5-HT) have been reported in T2DM patients, though the potential effect on insulin secretion is unclear. However, it is known that the 5-HT receptor 2C (5-HT(2C)R) agonist, mCPP, decreases plasma insulin concentration in mice. As such, we aimed to investigate the expression of the 5-HT(2C)R in pancreatic islets of diabetic mice and the role of 5-HT(2C)R signaling in insulin secretion from pancreatic β-cells. We found that 5-HT(2C)R expression was significantly increased in pancreatic islets of db/db mice. Furthermore, treatment with a 5-HT(2C)R antagonist (SB242084) increased insulin secretion from pancreatic islets isolated from db/db mice in a dose-dependent manner, but had no effect in islets from control mice. The effect of a 5-HT(2C)R agonist (mCPP) and antagonist (SB242084) were further studied in isolated pancreatic islets from mice and Min-6 cells. We found that mCPP significantly inhibited insulin secretion in Min-6 cells and isolated islets in a dose-dependent manner, which could be reversed by SB242084 or RNA interference against 5-HT(2C)R. We also treated Min-6 cells with palmitic acid for 24 h, and found that the expression of 5-HT(2C)R increased in a dose-dependent manner; furthermore, the inhibition of insulin secretion in Min-6 cells induced by palmitic acid could be reversed by SB242084 or RNA interference against 5-HT(2C)R. Taken together, our data suggests that increased expression of 5-HT(2C)R in pancreatic β-cells might inhibit insulin secretion. This unique observation increases our understanding of T2DM and suggests new avenues for potential treatment."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0054250"xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/author | "Gao L."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/author | "Han X."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/author | "Zhang Q."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/author | "Yuan L."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/author | "Zhu Y."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/author | "Zhou W."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/pages | "e54250"xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/title | "Serotonin receptor 2C and insulin secretion."xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/23349838 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23349838 |
| http://purl.uniprot.org/citations/23349838 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23349838 |
| http://purl.uniprot.org/uniprot/#_D0FZQ3-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_A2AV66-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_B1ATN4-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_B2RUD4-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_P48356-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_Q3UNU8-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_Q8BZI5-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_P34968-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |
| http://purl.uniprot.org/uniprot/#_Q3US58-mappedCitation-23349838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23349838 |