| http://purl.uniprot.org/citations/23371559 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23371559 | http://www.w3.org/2000/01/rdf-schema#comment | "Rice seed has been used as a production platform for high value recombinant proteins. When mature human interleukin 7 (hIL-7) was expressed as a secretory protein in rice endosperm by ligating the N terminal glutelin signal peptide and the C terminal KDEL endoplasmic reticulum (ER) retention signal to the hIL-7 cytokine to improve production yield, this protein accumulated at levels visible by Coomassie Brilliant Blue staining. However, the production of this protein led not only to a severe reduction of endogenous seed storage proteins but also to a deterioration in grain quality. The appearance of aberrant grain phenotypes (such as floury and shrunken) was attributed to ER stress induced by the retention of highly aggregated unfolded hIL-7 in the ER lumen, and the expression levels of chaperones such as BiPs and PDIs were enhanced in parallel with the increase in hIL-7 levels. The activation of this ER stress response was shown to be mainly mediated by the OsIRE1-OsbZIP50 signal cascade, based on the appearance of unconventional splicing of OsbZIP50 mRNA and the induction of OsBiP4&5. Interestingly, the ER stress response could be induced by lower concentrations of hIL-7 versus other types of cytokines such as IL-1b, IL-4, IL-10, and IL-18. Furthermore, several ubiquitin 26S proteasome-related genes implicated in ER-associated degradation were upregulated by hIL-7 production. These results suggest that severe detrimental effects on grain properties were caused by proteo-toxicity induced by unfolded hIL-7 aggregates in the ER, resulting in the triggering of ER stress."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11103-013-0016-5"xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/author | "Ohta M."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/author | "Takahashi S."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/author | "Yang L."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/author | "Takaiwa F."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/author | "Kudo K."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/author | "Wakasa Y."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/name | "Plant Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/pages | "461-475"xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/title | "ER stress response induced by the production of human IL-7 in rice endosperm cells."xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://purl.uniprot.org/core/volume | "81"xsd:string |
| http://purl.uniprot.org/citations/23371559 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23371559 |
| http://purl.uniprot.org/citations/23371559 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23371559 |
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| http://purl.uniprot.org/uniprot/#_Q7XIT1-mappedCitation-23371559 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23371559 |
| http://purl.uniprot.org/uniprot/Q75HQ0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23371559 |
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