| http://purl.uniprot.org/citations/23372835 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23372835 | http://www.w3.org/2000/01/rdf-schema#comment | "The passage of leukocytes across the endothelium and into arterial walls is a critical step in the development of atherosclerosis. Previously, we showed in vitro that the RhoG guanine nucleotide exchange factor SGEF (Arhgef26) contributes to the formation of ICAM-1-induced endothelial docking structures that facilitate leukocyte transendothelial migration. To further explore the in vivo role of this protein during inflammation, we generated SGEF-deficient mice. When crossed with ApoE null mice and fed a Western diet, mice lacking SGEF showed a significant decrease in the formation of atherosclerosis in multiple aortic areas. A fluorescent biosensor revealed local activation of RhoG around bead-clustered ICAM-1 in mouse aortic endothelial cells. Notably, this activation was decreased in cells from SGEF-deficient aortas compared to wild type. In addition, scanning electron microscopy of intimal surfaces of SGEF(-/-) mouse aortas revealed reduced docking structures around beads that were coated with ICAM-1 antibody. Similarly, under conditions of flow, these beads adhered less stably to the luminal surface of carotid arteries from SGEF(-/-) mice. Taken together, these results show for the first time that a Rho-GEF, namely SGEF, contributes to the formation of atherosclerosis by promoting endothelial docking structures and thereby retention of leukocytes at athero-prone sites of inflammation experiencing high shear flow. SGEF may therefore provide a novel therapeutic target for inhibiting the development of atherosclerosis."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0055202"xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Burridge K."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Hahn K."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Kroon J."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Samson T."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "van Buul J.D."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Welch C."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "van den Berg T.K."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Sharek L."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Bakker E.N."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Matlung H.L."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/author | "Doerschuk C."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/pages | "e55202"xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/title | "The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis."xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/23372835 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23372835 |
| http://purl.uniprot.org/citations/23372835 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23372835 |
| http://purl.uniprot.org/uniprot/P13597#attribution-20E6C22E067790536F3ADBB93C535AC8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23372835 |
| http://purl.uniprot.org/uniprot/P84096#attribution-20E6C22E067790536F3ADBB93C535AC8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23372835 |
| http://purl.uniprot.org/uniprot/D3YYY8#attribution-93FE97F8EF23AFE4E7DF752EB8BF5D48 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23372835 |
| http://purl.uniprot.org/uniprot/F6TAL7#attribution-93FE97F8EF23AFE4E7DF752EB8BF5D48 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23372835 |