| http://purl.uniprot.org/citations/23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23396200 | http://www.w3.org/2000/01/rdf-schema#comment | "Notch signaling plays a pivotal role in embryonic and postnatal development. Upon binding of a Notch ligand, proteolytic cleavage events liberate the Notch-intracellular domain (NICD) that migrates into the nucleus. In order to activate target genes, NICD associates with the transcription factor RBP-J (also known as CSL), Mastermind and the acetyltransferase p300. Here, we identify the DEAD-box RNA helicase Ddx5 as a novel component of the RBP-J/NICD complex utilizing a biotinylation-tagging approach followed by mass-spectrometry. Biochemical assays confirm a direct interaction of Ddx5 with RBP-J. We show that Ddx5 localizes at RBP-J binding sites within the Notch target genes preTCRα, Hes1 and CD25 in a Notch-dependent manner. Moreover, knockdown of Ddx5 also downregulates a subset of Notch target genes in a murine pre T-cell model. Interestingly, also knockdown/overexpression of the RNA coactivator SRA, a cofactor of Ddx5, downregulates Hes1 and preTCRα. Using Chromatin-IP, we show that this effect is accompanied with a loss of p300 occupancy at Notch target genes and decreased histone acetylation. Together, our data demonstrate that Ddx5 and SRA function as coactivators of Notch signaling."xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2013.01.032"xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/author | "Jung C."xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/author | "Mittler G."xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/author | "Borggrefe T."xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/author | "Oswald F."xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/pages | "1180-1189"xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/title | "RNA helicase Ddx5 and the noncoding RNA SRA act as coactivators in the Notch signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://purl.uniprot.org/core/volume | "1833"xsd:string |
| http://purl.uniprot.org/citations/23396200 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23396200 |
| http://purl.uniprot.org/citations/23396200 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23396200 |
| http://purl.uniprot.org/uniprot/#_A1L333-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_A0A8Q0Q6H9-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_A0A494BBA2-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_B1ARB9-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_B1ARC0-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_G3UZQ4-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_G3X8R2-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_Q3UXW3-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_Q3V0Z8-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_Q921Y3-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |
| http://purl.uniprot.org/uniprot/#_O35662-mappedCitation-23396200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23396200 |