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http://purl.uniprot.org/citations/23496987http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23496987http://www.w3.org/2000/01/rdf-schema#comment"

Background

MicroRNAs (miRNAs) emerge as new important regulators of lipid homeostasis by regulating corresponding genes. MiR-613 is a newly discovered microRNA, of which the biological function is unknown. A recent report has shown that miR-613 downregulates liver X receptor α (LXRα), a ligand-activated nuclear receptor playing an important role in the regulation of lipid metabolism. The purpose of this study is to explore the effect and the molecular basis of miR-613 on lipogenesis in HepG2 cells.

Methods

HepG2 cells were transiently transfected with miR-613 mimic or control microRNA. Real time PCR, Western blot, Luciferase reporter assay and Oil Red O staining were employed to examine the expression of LXRα and its target genes involved in lipogenesis, binding site for miR-613 in 3'-untranslated region (3'-UTR) of LXRα mRNA and lipid droplet accumulation in the cells.

Results

MiR-613 dramatically suppressed the expression of LXRα and its target genes including sterol-regulatory element binding protein 1c (SREBP-1c), fatty acid synthase (FAS), carbohydrate responsive element-binding protein (ChREBP) and acetyl-CoA carboxylase (ACC). Reporter assay showed that miR-613 directly bound to 3'-UTR of LXRα mRNA. Moreover, miR-613 significantly repressed LXRα-induced lipid droplet accumulation in HepG2 cells. Ectopic expression of LXRα without 3'-UTR markedly attenuated the miR-613-mediated downregulation of LXRα's target genes and LXRα-induced lipid droplet accumulation.

Conclusions

MiR-613 suppresses lipogenesis by directly targeting LXRα in HepG2 cells, suggesting that miR-613 may serve as a novel target for regulating lipid homeostasis."xsd:string
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http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Huang G."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Gao M."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Zhang Y."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Hu C.J."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Zhong D."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"He F.T."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Zeng Y.J."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/author"Wu G.Z."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/name"Lipids Health Dis"xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/pages"32"xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/title"MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRalpha."xsd:string
http://purl.uniprot.org/citations/23496987http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/23496987http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23496987
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