| http://purl.uniprot.org/citations/23520014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23520014 | http://www.w3.org/2000/01/rdf-schema#comment | "Overexpression of matrix metalloproteinase (MMP)-3 and -13 can lead to the dedifferentiation of expanded chondrocytes. After implanting dedifferentiated cells for cartilage defect repair, graft failure may occur. Short hairpin RNA (shRNA) is a powerful genetic tool to reduce the expression of target genes. This study investigated the effects of chitosan-plasmid DNA (pDNA) nanoparticles encoding shRNA targeting MMP-3 and -13 on the dedifferentiation of expanded chondrocytes. The objective was to optimize the parameters of chitosan-pDNA formulation for achieving higher efficiency of pDNA delivery and gene silencing. The chitosan-pDNA nanoparticles were prepared using a complex coacervation process. Then the characteristics including size, shape, stability, and transfection efficiency were compared in different groups. The results indicated that chitosan of 800 kDa at N/P ratio of 4 and pH 7.0 was optimal to prepare chitosan-pDNA nanoparticles. These nanoparticles showed high DNA loading efficiency (95.8 ± 1.5%) and high gene transfection efficiency (24.5 ± 1.6%). After the expanded chondrocytes were transfected by chitosan-pDNA nanoparticles, MMP-3-610 and MMP-13-2024 groups showed greater suppression in mRNA and protein levels. The results indicated that chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.org/dc/terms/identifier | "doi:10.1002/jbm.a.34711"xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Fan X."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Zhang Q."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Zhang C."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Sun F."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Fan H."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/author | "Xiong C."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/name | "J Biomed Mater Res A"xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/pages | "373-380"xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/title | "Chitosan-plasmid DNA nanoparticles encoding small hairpin RNA targeting MMP-3 and -13 to inhibit the expression of dedifferentiation related genes in expanded chondrocytes."xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://purl.uniprot.org/core/volume | "102"xsd:string |
| http://purl.uniprot.org/citations/23520014 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23520014 |
| http://purl.uniprot.org/citations/23520014 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23520014 |
| http://purl.uniprot.org/uniprot/#_P28863-mappedCitation-23520014 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23520014 |
| http://purl.uniprot.org/uniprot/P28863 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23520014 |