| http://purl.uniprot.org/citations/23533611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23533611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23533611 | http://www.w3.org/2000/01/rdf-schema#comment | "Cardiolipin, a major component of mitochondria, is critical for mitochondrial functioning including the regulation of cytochrome c release during apoptosis and proper electron transport. Mitochondrial cardiolipin with its unique bulky amphipathic structure is a potential substrate for phospholipase A2 (PLA2) in vivo. We have developed mass spectrometric methodology for analyzing PLA2 activity toward various cardiolipin forms and demonstrate that cardiolipin is a substrate for sPLA2, cPLA2 and iPLA2, but not for Lp-PLA2. Our results also show that none of these PLA2s have significant PLA1 activities toward dilyso-cardiolipin. To understand the mechanism of cardiolipin hydrolysis by PLA2, we also quantified the release of monolyso-cardiolipin and dilyso-cardiolipin in the PLA2 assays. The sPLA2s caused an accumulation of dilyso-cardiolipin, in contrast to iPLA2 which caused an accumulation of monolyso-cardiolipin. Moreover, cardiolipin inhibits iPLA2 and cPLA2, and activates sPLA2 at low mol fractions in mixed micelles of Triton X-100 with the substrate 1-palmitoyl-2-arachidonyl-sn-phosphtidylcholine. Thus, cardiolipin functions as both a substrate and a regulator of PLA2 activity and the ability to assay the various forms of PLA2 is important in understanding its function."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0059267"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0059267"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Cao J."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Cao J."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Dumlao D.S."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Dumlao D.S."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Dennis E.A."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Dennis E.A."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Hsu Y.H."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/author | "Hsu Y.H."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/pages | "E59267"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/pages | "E59267"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/title | "Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/title | "Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/23533611 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23533611 |
| http://purl.uniprot.org/citations/23533611 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23533611 |