| http://purl.uniprot.org/citations/23535175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23535175 | http://www.w3.org/2000/01/rdf-schema#comment | "The DEAD box RNA helicase Rck and the scaffold protein Pat1b participate in controlling gene expression at the post-transcriptional level by suppressing mRNA translation and promoting mRNA decapping. In addition, both proteins are required for the assembly of processing (P)-bodies, cytoplasmic foci that contain stalled mRNAs and numerous components of the mRNA decay machinery. The C-terminal RecA-like domain of Rck interacts with the N-terminal acidic domain of Pat1b. Here, we identified point mutations in human Rck and Pat1b that prevent the two proteins from binding to each other. By analyzing interaction-deficient mutants in combination with knockdown and rescue strategies in human HeLa cells, we found that Pat1b assembles P-bodies and suppresses expression of tethered mRNAs in the absence of Rck binding. In contrast, Rck requires the Pat1b-binding site in order to promote P-body assembly and associate with the decapping enzyme Dcp2 as well as Ago2 and TNRC6A, two core components of the RNA-induced silencing complex. Our data indicate that P-body assembly occurs in a step-wise manner, where Rck participates in the initial suppression of mRNA translation, whereas Pat1b in a second step triggers P-body assembly and promotes mRNA decapping."xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.org/dc/terms/identifier | "doi:10.4161/rna.24086"xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/author | "Stoecklin G."xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/author | "Ozgur S."xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/name | "RNA Biol"xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/pages | "528-539"xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/title | "Role of Rck-Pat1b binding in assembly of processing-bodies."xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/23535175 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23535175 |
| http://purl.uniprot.org/citations/23535175 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23535175 |
| http://purl.uniprot.org/uniprot/#_B2R858-mappedCitation-23535175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23535175 |
| http://purl.uniprot.org/uniprot/#_B7Z1D3-mappedCitation-23535175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23535175 |
| http://purl.uniprot.org/uniprot/#_B3KXN0-mappedCitation-23535175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23535175 |
| http://purl.uniprot.org/uniprot/#_P26196-mappedCitation-23535175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23535175 |
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| http://purl.uniprot.org/uniprot/B2R858 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23535175 |