| http://purl.uniprot.org/citations/23545304 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23545304 | http://www.w3.org/2000/01/rdf-schema#comment | "In response to severe bacterial infection, bone marrow hematopoietic activity shifts toward promoting granulopoiesis. The underlying cell signaling mechanisms remain obscure. To study the role of Toll-like receptor 4 (TLR4)/stem cell antigen-1 (Sca-1) signaling in this process, bacteremia was induced in mice by intravenous injection of Escherichia coli. A subgroup of animals also received intravenous 5-bromo-2-deoxyuridine (BrdU). In a separate set of experiments, bone marrow lineage-negative (lin(-)) stem cell growth factor receptor-positive (c-kit(+)) Sca-1(-) cells containing primarily common myeloid progenitors were cultured in vitro without or with E. coli lipopolysaccharide (LPS). In genotypic background control mice, bacteremia significantly upregulated Sca-1 expression by lin(-) c-kit(+) cells, as reflected by a marked increase in BrdU-negative lin(-) c-kit(+) Sca-1(+) cells in the bone marrow. In mice with the TLR4 gene deletion, this bacteremia-evoked Sca-1 response was blocked. In vitro, LPS induced a dose-dependent increase in Sca-1 expression by cultured marrow lin(-) c-kit(+) Sca-1(-) cells. LPS-induced upregulation of Sca-1 expression was regulated at the transcriptional level. Inhibition of c-Jun N-terminal kinase/stress-activated protein kinase (JNK) activity with the specific inhibitor SP600125 suppressed LPS-induced upregulation of Sca-1 expression by marrow lin(-) c-kit(+) Sca-1(-) cells. Engagement of Sca-1 with anti-Sca-1 antibodies enhanced the expression of Sfpi1 spleen focus-forming virus (SFFV) proviral integration 1 (PU.1) in marrow lin(-) c-kit(+) Sca-1(-) cells cultured with LPS. Sca-1 null mice failed to maintain the marrow pool of granulopoietic cells following bacteremia. These results demonstrate that TLR4/Sca-1 signaling plays an important role in the regulation of hematopoietic precursor cell programming and their enhancement of granulocyte lineage commitment in response to E. coli bacteremia."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.01280-12"xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/author | "Shi X."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/author | "Zhang P."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/author | "Stanford W.L."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/author | "Basson M.D."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/author | "Melvan J.N."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/author | "Siggins R.W."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/name | "Infect Immun"xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/pages | "2197-2205"xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/title | "Toll-like receptor 4/stem cell antigen 1 signaling promotes hematopoietic precursor cell commitment to granulocyte development during the granulopoietic response to Escherichia coli bacteremia."xsd:string |
| http://purl.uniprot.org/citations/23545304 | http://purl.uniprot.org/core/volume | "81"xsd:string |
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