| http://purl.uniprot.org/citations/23562655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23562655 | http://www.w3.org/2000/01/rdf-schema#comment | "Glioblastoma multiforme (GBM) is the most common malignant brain tumor, which, despite combined modality treatment, reoccurs and is invariably fatal for affected patients. Recently, a member of the serine/threonine protein kinase D (PRKD) family, PRKD2, was shown to be a potent mediator of glioblastoma growth. Here we studied the role of PRKD2 in U87MG glioblastoma cell migration and invasion in response to sphingosine-1-phosphate (S1P), an activator of PRKD2 and a GBM mitogen. Time-lapse microscopy demonstrated that random cell migration was significantly diminished in response to PRKD2 silencing. The pharmacological PRKD family inhibitor CRT0066101 decreased chemotactic migration and invasion across uncoated or matrigel-coated Transwell inserts. Silencing of PRKD2 attenuated migration and invasion of U87MG cells even more effectively. In terms of downstream signaling, CRT0066101 prevented PRKD2 autophosphorylation and inhibited p44/42 MAPK and to a smaller extent p54/46 JNK and p38 MAPK activation. PRKD2 silencing impaired activation of p44/42 MAPK and p54/46 JNK, downregulated nuclear c-Jun protein levels and decreased c-Jun(S73) phosphorylation without affecting the NFκB pathway. Finally, qPCR array analyses revealed that silencing of PRKD2 downregulates mRNA levels of integrin alpha-2 and -4 (ITGA2 and -4), plasminogen activator urokinase (PLAU), plasminogen activator urokinase receptor (PLAUR), and matrix metallopeptidase 1 (MMP1). Findings of the present study identify PRKD2 as a potential target to interfere with glioblastoma cell migration and invasion, two major determinants contributing to recurrence of glioblastoma after multimodality treatment."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2013.03.029"xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Zimmer A."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Bernhart E."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Sattler W."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Wintersperger A."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Raynham T."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Damm S."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "DeVaney T."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/author | "Ireson C."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/name | "Exp Cell Res"xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/pages | "2037-2048"xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/title | "Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro."xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://purl.uniprot.org/core/volume | "319"xsd:string |
| http://purl.uniprot.org/citations/23562655 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23562655 |
| http://purl.uniprot.org/citations/23562655 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23562655 |
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| http://purl.uniprot.org/uniprot/#_B4E125-mappedCitation-23562655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23562655 |
| http://purl.uniprot.org/uniprot/#_P05412-mappedCitation-23562655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23562655 |
| http://purl.uniprot.org/uniprot/#_Q9BZL6-mappedCitation-23562655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23562655 |
| http://purl.uniprot.org/uniprot/#_Q8N2H2-mappedCitation-23562655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23562655 |
| http://purl.uniprot.org/uniprot/B4E125 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23562655 |
| http://purl.uniprot.org/uniprot/A0JLT6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23562655 |