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http://purl.uniprot.org/citations/23564079http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23564079http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To investigate whether sequestosome 1/p62 (p62), a key cargo adaptor protein involved in both the ubiquitin-proteasome system and the autophagy-lysosome system, could directly regulate autophagy in vitro.

Methods

HEK 293 cells or HeLa cells were transfected with p62-expressing plasmids or siRNA targeting p62. The cells or the cell lysates were subsequently subjected to immunofluorescence assay, immunoprecipitation assay, or immunoblot analysis. In vitro pulldown assay was used to study the interaction of p62 with Bcl-2.

Results

Overexpression of p62 significantly increased the basal level of autophagy in both HEK 293 cells and HeLa cells, whereas knockdown of p62 significantly decreased the basal level of autophagy. In vitro pulldown assay showed that p62 directly interacted with Bcl-2. It was observed in HeLa cells that p62 co-localized with Bcl-2. Furthermore, knockdown of p62 in HEK 293 cells significantly increased the amount of Beclin 1 that co-immunoprecipitated with Bcl-2.

Conclusion

p62 induces autophagy by disrupting the association between Bcl-2 and Beclin 1."xsd:string
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http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Chen D."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Gao F."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Zhou L."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Wang G.H."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Wang H.F."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Fu C."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Ying Z."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Xu R.J."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Ren H.G."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/author"Hu Q.S."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/name"Acta Pharmacol Sin"xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/pages"651-656"xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/title"Bcl-2-dependent upregulation of autophagy by sequestosome 1/p62 in vitro."xsd:string
http://purl.uniprot.org/citations/23564079http://purl.uniprot.org/core/volume"34"xsd:string
http://purl.uniprot.org/citations/23564079http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23564079
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