http://purl.uniprot.org/citations/23593298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23593298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23593298 | http://www.w3.org/2000/01/rdf-schema#comment | "Glutathione (GSH) and GSH-dependent enzymes play a key role in cellular detoxification processes that enable organism to cope with various internal and environmental stressors. However, it is often not clear, which components of the complex GSH-metabolism are required for tolerance towards a certain stressor. To address this question, a small scale RNAi-screen was carried out in Caenorhabditis elegans where GSH-related genes were systematically knocked down and worms were subsequently analysed for their survival rate under sub-lethal concentrations of arsenite and the redox cycler juglone. While the knockdown of γ-glutamylcysteine synthetase led to a diminished survival rate under arsenite stress conditions, GSR-1 (glutathione reductase) was shown to be essential for survival under juglone stress conditions. gsr-1 is the sole GSR encoding gene found in C. elegans. Knockdown of GSR-1 hardly affected total glutathione levels nor reduced glutathione/glutathione disulphide (GSH/GSSG) ratio under normal laboratory conditions. Nevertheless, when GSSG recycling was impaired by gsr-1(RNAi), GSH synthesis was induced, but not vice versa. Moreover, the impact of GSSG recycling was potentiated under oxidative stress conditions, explaining the enormous effect gsr-1(RNAi) knockdown had on juglone tolerance. Accordingly, overexpression of GSR-1 was capable of increasing stress tolerance. Furthermore, expression levels of SKN-1-regulated GSR-1 also affected life span of C. elegans, emphasising the crucial role the GSH redox state plays in both processes."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0060731"xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0060731"xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Daniel J."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Daniel J."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Liebau E."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Liebau E."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Lueersen K."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Lueersen K."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Ajonina C."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Ajonina C."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Ajonina I."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Ajonina I."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Drescher M."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Drescher M."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Hertel P."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Hertel P."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Stegehake D."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Stegehake D."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Woltersdorf C."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/author | "Woltersdorf C."xsd:string |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
http://purl.uniprot.org/citations/23593298 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |