| http://purl.uniprot.org/citations/23601700 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23601700 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, we have shown that a proportion of the matrix metalloproteinase-9 (MMP-9) synthesized by the macrophage cell line THP-1 binds to a chondroitin sulfate proteoglycan (CSPG) core protein to form a reduction-sensitive heteromer. It was also shown that the hemopexin-like (PEX) domain and the fibronectin-like (FnII) module in the enzyme are involved in heteromer formation. In this paper, we show that reduction-sensitive and SDS-stable heteromers may be reconstituted in vitro by mixing proMMP-9 with either serglycin, versican or CSPGs isolated from various monocytic cell lines. In addition, a strong but SDS-soluble proMMP-9·CSPG heteromer was formed. The two macromolecules in the SDS-stable reduction-sensitive heteromers were not linked together by disulfide bonds. As for the heteromer isolated from THP-1 cells, in vitro reconstituted SDS-stable and SDS-soluble heteromers showed weaker binding to gelatin than the proMMP-9 monomer. Furthermore, gelatin inhibited in vitro reconstitution of the heteromers, showing that the FnII module is involved in the complex formation. Tissue inhibitor of metalloproteinase (TIMP)-1 was not be detected in the proMMP-9·CSPG complexes. However, the presence of TIMP-1 inhibited formation of the SDS-soluble heteromer, but not the SDS-stable reduction-sensitive heteromer. This indicates that different regions in the PEX domain are involved formation of these heteromers."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.12291"xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/author | "Berg E."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/author | "Svineng G."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/author | "Theocharis A.D."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/author | "Uhlin-Hansen L."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/author | "Winberg J.O."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/author | "Malla N."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/name | "FEBS J"xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/pages | "2870-2887"xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/title | "In vitro reconstitution of complexes between pro-matrix metalloproteinase-9 and the proteoglycans serglycin and versican."xsd:string |
| http://purl.uniprot.org/citations/23601700 | http://purl.uniprot.org/core/volume | "280"xsd:string |
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