| http://purl.uniprot.org/citations/23603117 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23603117 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23603117 | http://www.w3.org/2000/01/rdf-schema#comment | "In Saccharomyces cerevisiae and higher eukaryotes, the loading of the replicative helicase MCM2-7 onto DNA requires the combined activities of ORC, Cdc6, and Cdt1. These proteins load MCM2-7 in an unknown way into a double hexamer around DNA. Here we show that MCM2-7 recruitment by ORC/Cdc6 is blocked by an autoinhibitory domain in the C terminus of Mcm6. Interestingly, Cdt1 can overcome this inhibitory activity, and consequently the Cdt1-MCM2-7 complex activates ORC/Cdc6 ATP-hydrolysis to promote helicase loading. While Cdc6 ATPase activity is known to facilitate Cdt1 release and MCM2-7 loading, we discovered that Orc1 ATP-hydrolysis is equally important in this process. Moreover, we found that Orc1/Cdc6 ATP-hydrolysis promotes the formation of the ORC/Cdc6/MCM2-7 (OCM) complex, which functions in MCM2-7 double-hexamer assembly. Importantly, CDK-dependent phosphorylation of ORC inhibits OCM establishment to ensure once per cell cycle replication. In summary, this work reveals multiple critical mechanisms that redefine our understanding of DNA licensing."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2013.03.026"xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2013.03.026"xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Winkler C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Winkler C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Evrin C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Evrin C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Fernandez-Cid A."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Fernandez-Cid A."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Gardenal E."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Gardenal E."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Herrera M.C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Herrera M.C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Riera A."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Riera A."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Samel S."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Samel S."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Speck C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Speck C."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Tognetti S."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Tognetti S."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Uhle S."xsd:string |
| http://purl.uniprot.org/citations/23603117 | http://purl.uniprot.org/core/author | "Uhle S."xsd:string |