| http://purl.uniprot.org/citations/23625312 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23625312 | http://www.w3.org/2000/01/rdf-schema#comment | "Flotillins are membrane association proteins consisting of two homologous members, flotillin-1 (Flot-1) and flotillin-2 (Flot-2). They define a clathrin-independent endocytic pathway in mammal cells, which are also distinct from some other endocytosis mechanisms. The implicated cargoes of the flotillin-dependent pathway are mainly some GPI-anchored proteins, such as CD59 and Thy-1, which positionally colocalize with flotillins at the plasma membrane microdomains. To see whether flotillins are involved in the endocytosis of PrP(C), the potential molecular interaction between PrP(C) and flotillins in a neuroblastoma cell line SK-N-SH was analyzed. Co-immunoprecipitation assays did not reveal a detectable complex in the cell lysates of a normal feeding situation. After stimulation of Cu(2+), PrP(C) formed a clear complex with Flot-1, but not with Flot-2. Immunofluorescent assays illustrated that PrP(C) colocalized well with Flot-1, and the complexes of PrP(C)-Flot-1 shifted from the cell membrane to the cytoplasm along with the treatment of Cu(2+). Down-regulating the expression of Flot-1 in SK-N-SH cells by Flot-1-specific RNAi obviously abolished the Cu(2+)-stimulated endocytosis process of PrP(C). Moreover, we also found that in the cell line human embryonic kidney 293 (HEK293) without detectable PrP(C) expression, the distribution of cellular Flot-1 maintained almost unchanged during Cu(2+) treatment. Cu(2+)-induced PrP(C)-Flot-1 molecular interaction and endocytosis in HEK293 cells were obtained when expressing wild-type human PrP (PrP(PG5)), but not in the preparation expressing octarepeat-deleted PrP (PrP(PG0)). Our data here provide direct evidences for the molecular interaction and endocytosis of PrP(C) with Flot-1 in the presence of copper ions, and the octarepeat region of PrP(C) is critical for this process, which strongly indicates that the Flot-1-dependent endocytic pathway seems to mediate the endocytosis process of PrP(C) in the special situation."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.org/dc/terms/identifier | "doi:10.1007/s12035-013-8452-4"xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Gao C."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Shi Q."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Wang K."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Tian C."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Dong X.P."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Ren K."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/author | "Wang S.B."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/name | "Mol Neurobiol"xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/pages | "631-646"xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/title | "Flotillin-1 mediates PrPc endocytosis in the cultured cells during Cu^2⁺ stimulation through molecular interaction."xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/23625312 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23625312 |
| http://purl.uniprot.org/citations/23625312 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23625312 |
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| http://purl.uniprot.org/uniprot/#_A2VCL5-mappedCitation-23625312 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23625312 |