| http://purl.uniprot.org/citations/23628981 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23628981 | http://www.w3.org/2000/01/rdf-schema#comment | "Leukemia inhibitor factor (LIF) is a three disulfide bridge-containing cytokine with numerous regulatory effects. In this report, we present the high level expression of a soluble recombinant human LIF (rhLIF) in Escherichia coli. A codon-optimized Profinity eXact™-tagged hLIF cDNA was cloned into pET3b vector, and transformed into E. coli OrigamiB(DE3) harboring the bacterial thioredoxin coexpression vector. By using an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™), the yield of soluble proteins was significantly improved in comparison to commonly-used 2 × YT media. The recombinant protein was purified via a single chromatographic step using an affinity tag-based protein purification system that processed by cleavage with sodium fluoride, resulting in the complete proteolytic removal the N-terminal tag. Soluble rhLIF yield was estimated to be approximately 1mg/g of wet weight cells, with above 98% purity. The rhLIF protein specifically inhibited the proliferation of the murine myeloblastic leukemia M1 cell in a dose-dependent manner, and induced Stat3 phosphorylation in mouse ES cells. This novel expression and purification protocol for the production of recombinant hLIF is a simple, suitable, and effective method."xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2013.04.006"xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/author | "Nishikawa S."xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/author | "Imaizumi K."xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/author | "Akuta T."xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/author | "Tarui H."xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/name | "Protein Expr Purif"xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/pages | "20-26"xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/title | "High-level expression and efficient one-step chromatographic purification of a soluble human leukemia inhibitory factor (LIF) in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://purl.uniprot.org/core/volume | "90"xsd:string |
| http://purl.uniprot.org/citations/23628981 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23628981 |
| http://purl.uniprot.org/citations/23628981 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23628981 |
| http://purl.uniprot.org/uniprot/#_P15018-mappedCitation-23628981 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23628981 |
| http://purl.uniprot.org/uniprot/P15018 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23628981 |