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http://purl.uniprot.org/citations/23637930http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23637930http://www.w3.org/2000/01/rdf-schema#comment"

Aims

In collateral development (i.e. arteriogenesis), mononuclear cells are important and exist as a heterogeneous population consisting of pro-inflammatory and anti-inflammatory/repair-associated cells. Protease-activated receptor (PAR)1 and PAR2 are G-protein-coupled receptors that are both expressed by mononuclear cells and are involved in pro-inflammatory reactions, while PAR2 also plays a role in repair-associated responses. Here, we investigated the physiological role of PAR1 and PAR2 in arteriogenesis in a murine hind limb ischemia model.

Methods and results

PAR1-deficient (PAR1-/-), PAR2-deficient (PAR2-/-) and wild-type (WT) mice underwent femoral artery ligation. Laser Doppler measurements revealed reduced post-ischemic blood flow recovery in PAR2-/- hind limbs when compared to WT, while PAR1-/-mice were not affected. Upon ischemia, reduced numbers of smooth muscle actin (SMA)-positive collaterals and CD31-positive capillaries were found in PAR2-/-mice when compared to WT mice, whereas these parameters in PAR1-/-mice did not differ from WT mice. The pool of circulating repair-associated (Ly6C-low) monocytes and the number of repair-associated (CD206-positive) macrophages surrounding collaterals in the hind limbs were increased in WT and PAR1-/- mice, but unaffected in PAR2-/-mice. The number of repair-associated macrophages in PAR2-/-hind limbs correlated with CD11b- and CD115-expression on the circulating monocytes in these animals, suggesting that monocyte extravasation and M-CSF-dependent differentiation into repair-associated cells are hampered.

Conclusion

PAR2, but not PAR1, is involved in arteriogenesis and promotes the repair-associated response in ischemic tissues. Therefore, PAR2 potentially forms a new pro-arteriogenic target in coronary artery disease (CAD) patients."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0061923"xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"van den Hengel L.G."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Reitsma P.H."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"van Zonneveld A.J."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"de Vries M.R."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Spek C.A."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"de Boer H.C."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Versteeg H.H."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Quax P.H."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Nossent A.Y."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Hamming J.F."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"van Oeveren-Rietdijk A.M."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/author"Hellingman A.A."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/pages"e61923"xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/title"Protease-activated receptor (PAR)2, but not PAR1, is involved in collateral formation and anti-inflammatory monocyte polarization in a mouse hind limb ischemia model."xsd:string
http://purl.uniprot.org/citations/23637930http://purl.uniprot.org/core/volume"8"xsd:string
http://purl.uniprot.org/citations/23637930http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23637930
http://purl.uniprot.org/citations/23637930http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/23637930
http://purl.uniprot.org/uniprot/#_P30558-mappedCitation-23637930http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23637930
http://purl.uniprot.org/uniprot/#_Q3TU81-mappedCitation-23637930http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23637930
http://purl.uniprot.org/uniprot/#_P55086-mappedCitation-23637930http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23637930
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