| http://purl.uniprot.org/citations/23640461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23640461 | http://www.w3.org/2000/01/rdf-schema#comment | "Development of drugs targeting Bcl-2 relatives and caspases, for treating diseases including cancer and inflammatory disorders, often involves measuring interactions with recombinant target molecules, and/or monitoring cancer cell killing in vitro. Here, we present yeast-based methods for evaluating drug-mediated inhibition of Bcl-2 relatives or caspases. Active Bax and caspases kill Saccharomyces cerevisiae, and pro-survival Bcl-2 proteins can inhibit Bax-induced yeast death. By measuring the growth or adenosine triphosphate content of transformants co-expressing Bax with pro-survival Bcl-2 relatives, we found that the Bcl-2 antagonist drugs ABT-737 or ABT-263 abolished Bcl-2 or Bcl-xL function and reduced Bcl-w activity, but failed to inhibit Mcl-1, A1 or the poxvirus orthologs DPV022 and SPPV14. Using this technique, we also demonstrated that adenoviral E1B19K was resistant to these agents. The caspase inhibitor Q-VD-OPh suppressed yeast death induced by caspases 1 and 3. Yeast engineered to express human apoptotic regulators enable simple, automatable assessment of the activity and specificity of candidate drugs targeting Bcl-2 relatives or caspases."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.org/dc/terms/identifier | "doi:10.1038/cddis.2013.143"xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/author | "Kvansakul M."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/author | "Hawkins C.J."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/author | "Kaur L."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/author | "Beaumont T.E."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/author | "Pantaki-Eimany D."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/author | "Shekhar T.M."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/name | "Cell Death Dis"xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/pages | "e619"xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/title | "Yeast techniques for modeling drugs targeting Bcl-2 and caspase family members."xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/23640461 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23640461 |
| http://purl.uniprot.org/citations/23640461 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23640461 |
| http://purl.uniprot.org/uniprot/#_P13090-mappedCitation-23640461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23640461 |
| http://purl.uniprot.org/uniprot/#_P33302-mappedCitation-23640461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23640461 |
| http://purl.uniprot.org/uniprot/#_P53049-mappedCitation-23640461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23640461 |
| http://purl.uniprot.org/uniprot/P53049 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23640461 |
| http://purl.uniprot.org/uniprot/P13090 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23640461 |
| http://purl.uniprot.org/uniprot/P33302 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23640461 |