| http://purl.uniprot.org/citations/23644595 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23644595 | http://www.w3.org/2000/01/rdf-schema#comment | "IscR from Escherichia coli is an unusual metalloregulator in that both apo and iron sulfur (Fe-S)-IscR regulate transcription and exhibit different DNA binding specificities. Here, we report structural and biochemical studies of IscR suggesting that remodeling of the protein-DNA interface upon Fe-S ligation broadens the DNA binding specificity of IscR from binding the type 2 motif only to both type 1 and type 2 motifs. Analysis of an apo-IscR variant with relaxed target-site discrimination identified a key residue in wild-type apo-IscR that, we propose, makes unfavorable interactions with a type 1 motif. Upon Fe-S binding, these interactions are apparently removed, thereby allowing holo-IscR to bind both type 1 and type 2 motifs. These data suggest a unique mechanism of ligand-mediated DNA site recognition, whereby metallocluster ligation relocates a protein-specificity determinant to expand DNA target-site selection, allowing a broader transcriptomic response by holo-IscR."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsmb.2568"xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/author | "Zwart P.H."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/author | "Brennan R.G."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/author | "Rajagopalan S."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/author | "Kiley P.J."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/author | "Phillips K.J."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/author | "Teter S.J."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/name | "Nat Struct Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/pages | "740-747"xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/title | "Studies of IscR reveal a unique mechanism for metal-dependent regulation of DNA binding specificity."xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/23644595 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23644595 |
| http://purl.uniprot.org/citations/23644595 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23644595 |
| http://purl.uniprot.org/uniprot/P0AGK8#attribution-B014725F9947A017AA4609516950A609 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23644595 |
| http://purl.uniprot.org/uniprot/#_P0AGK8-mappedCitation-23644595 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23644595 |
| http://purl.uniprot.org/uniprot/P0AGK8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23644595 |