http://purl.uniprot.org/citations/23648141 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23648141 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundT-cell factor (TCF) proteins represent key transcription factors that activate Wnt/β-catenin signalling. We have reported that a pair of TCF-4 isoforms (TCF-4C and TCF-4D) exhibit differential TCF transcriptional activity in hepatocellular carcinoma (HCC) cells, although their structure differs by only the presence (TCF-4D) or absence (TCF-4C) of exon 4.AimTo demonstrate a regulatory role of exon 4 in HCC development.MethodsTCF-4C and TCF-4D expression profiles were examined in 27 pairs of human HCC and adjacent liver tissues. The functional role of the TCF-4 isoforms was evaluated in OUMS-29 (an immortalized hepatocyte-derived) and HAK-1A (a well-differentiated HCC) cell lines using stable clones overexpressing the TCF-4 isoforms.ResultsTCF-4C was significantly upregulated in HCC tissues compared with corresponding peritumour and normal liver tissues; in contrast, there was no difference in TCF-4D expression. TCF-4C clones derived from both cell lines exhibited increased TCF activity, Wnt-responsive target genes, cell proliferation, cell cycle progression and resistance to chemotherapeutic drugs compared with TCF-4D clones. Capability of cell migration and colony formation was significantly higher in TCF-4C than TCF-4D clones. In a nude mice xenograft model, the HAK-1A-derived TCF-4C clone rapidly developed tumours compared with the TCF-4D clone. TCF-4C clone-derived tumours exhibited upregulation of Wnt-responsive target genes compared with the slow developing and small TCF-4D-derived tumours.ConclusionThese results demonstrate that the TCF-4C isoform lacking exon 4 is associated with a malignant phenotype compared with the exon 4-harbouring TCF-4D isoform, indicating that exon 4 of TCF-4 plays a prominent role in HCC development."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.org/dc/terms/identifier | "doi:10.1111/liv.12189"xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/author | "Kim M."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/author | "Yan T."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/author | "Wands J.R."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/author | "Xu C.Q."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/author | "Nambotin S.B."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/author | "Tomimaru Y."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/name | "Liver Int"xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/pages | "1536-1548"xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/title | "Loss of exon 4 in a human T-cell factor-4 isoform promotes hepatic tumourigenicity."xsd:string |
http://purl.uniprot.org/citations/23648141 | http://purl.uniprot.org/core/volume | "33"xsd:string |
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