| http://purl.uniprot.org/citations/23658651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23658651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23658651 | http://www.w3.org/2000/01/rdf-schema#comment | "The genetic features of the antimicrobial resistance of a multidrug resistant Klebsiella pneumoniae strain harboring bla NDM-1 were investigated to increase our understanding of the evolution of NDM-1. The strain, KPX, came from a Taiwanese patient with a hospitalization history in New Delhi. Complete DNA sequencing was performed; and the genes responsible for antimicrobial resistance were systematically examined and isolated by library screening. KPX harbored two resistance plasmids, pKPX-1 and pKPX-2, which are 250-kb and 141-kb in size, respectively, with bla NDM-1 present on pKPX-1. The plasmid pKPX-1 contained genes associated with the IncR and IncF groups, while pKPX-2 belonged to the IncF family. Each plasmid carried multiple antimicrobial resistance genetic determinants. The gene responsible for resistance to carbapenems was found on pKPX-1 and that for resistance to aztreonam was found on pKPX-2. To our surprise, we discovered that bla NDM-1 exists on pKPX-1 as multiple copies in the form of tandem repeats. Amplification of bla NDM-1 was found to occur by duplication of an 8.6-kb unit, with the copy number of the repeat varying from colony to colony. This repeat sequence is identical to that of the pNDM-MAR except for two base substitutions. The copy number of bla NDM-1 of colonies under different conditions was assessed by Southern blotting and quantitative PCR. The bla NDM-1 sequence was maintained in the presence of the antimicrobial selection; however, removal of antimicrobial selection led to the emergence of susceptible bacterial populations with a reduced copy number or even the complete loss of the bla NDM-1 sequence. The dynamic nature of the NDM-1 sequence provides a strong argument for judicious use of the broad-spectrum antimicrobials in order to reduce the development and spread of antimicrobial resistance among pathogens."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0062774"xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0062774"xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Tsai S.F."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Tsai S.F."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Wu K.M."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Wu K.M."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Kirby R."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Kirby R."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Liu Y.M."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Liu Y.M."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chang Y.H."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chang Y.H."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chen C.P."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chen C.P."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Lin A.C."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Lin A.C."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Lee Y.T."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Lee Y.T."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chang C.M."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chang C.M."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chen Y.T."xsd:string |
| http://purl.uniprot.org/citations/23658651 | http://purl.uniprot.org/core/author | "Chen Y.T."xsd:string |