| http://purl.uniprot.org/citations/23661684 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23661684 | http://www.w3.org/2000/01/rdf-schema#comment | "During RNA interference and related gene regulatory pathways, the endonuclease Dicer cleaves precursor RNA molecules to produce microRNAs (miRNAs) and short interfering RNAs (siRNAs). Human cells encode a single Dicer enzyme that can associate with two different double-stranded RNA (dsRNA)-binding proteins, protein activator of PKR (PACT) and trans-activation response RNA-binding protein (TRBP). However, the functional redundancy or differentiation of PACT and TRBP in miRNA and siRNA biogenesis is not well understood. Using a reconstituted system, we show here that PACT and TRBP have distinct effects on Dicer-mediated dsRNA processing. In particular, we found that PACT in complex with Dicer inhibits the processing of pre-siRNA substrates when compared with Dicer and a Dicer-TRBP complex. In addition, PACT and TRBP show non-redundant effects on the production of different-sized miRNAs (isomiRs), which in turn alter target-binding specificities. Experiments using chimeric versions of PACT and TRBP suggest that the two N-terminal RNA-binding domains of each protein confer the observed differences in dsRNA substrate recognition and processing behavior of Dicer-dsRNA-binding protein complexes. These results support the conclusion that in humans, Dicer-associated dsRNA-binding proteins are important regulatory factors that contribute both substrate and cleavage specificity during miRNA and siRNA production."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkt361"xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/author | "Doudna J.A."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/author | "Lee H.Y."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/author | "Smith A.M."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/author | "Zhou K."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/author | "Noland C.L."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/pages | "6568-6576"xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/title | "Differential roles of human Dicer-binding proteins TRBP and PACT in small RNA processing."xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://purl.uniprot.org/core/volume | "41"xsd:string |
| http://purl.uniprot.org/citations/23661684 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23661684 |
| http://purl.uniprot.org/citations/23661684 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23661684 |
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| http://purl.uniprot.org/uniprot/O75569#attribution-08A369C04C4545DEA6162388CD61ED81 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/23661684 |
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