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| http://purl.uniprot.org/citations/23675550 | http://www.w3.org/2000/01/rdf-schema#comment | "Infection with the intracellular protozoan parasite Leishmania mexicana causes chronic disease in C57BL/6 mice, in which cutaneous lesions persist for many months with high parasite burdens (10(7)-10(8) parasites). This chronic disease process requires host IL-10 and FcγRIII. When Leishmania amastigotes are released from cells, surface-bound IgG can induce IL-10 and suppress IL-12 production from macrophages. These changes decrease IFN-γ from T cells and nitric oxide production in infected cells, which are both required for Leishmania control. However, antibodies targets and the kinetics of antibody production are unknown. Several groups have been unsuccessful in identifying amastigote surface proteins that bind IgG. We now show that glycoinositol phospholipids (GIPLs) of L. mexicana are recognized by mouse IgG1 by 6 weeks of infection, with a rapid increase between 12 and 16 weeks, consistent with the timing of chronic disease in C57BL/6 mice vs. healing in FcγRIII-deficient mice. A single prominent spot on TLC is recognized by IgG, and the glycolipid is a glycosyl phosphatidylinositol containing a branched mannose structure. We show that the lipid structure of the GIPL (the sn-2 fatty acid) is required for antibody recognition. This GIPL is abundant in L. mexicana amastigotes, rare in stationary-phase promastigotes, and absent in L. major, consistent with a role for antibodies to GIPLs in chronic disease. A mouse monoclonal anti-GIPL IgG recognizes GIPLs on the parasite surface, and induces IL-10 from macrophages. The current work also extends this mouse analysis to humans, finding that L. mexicana-infected humans with localized and diffuse cutaneous leishmaniasis have antibodies that recognize GIPLs, can bind to the surface of amastigotes, and can induce IL-10 from human monocytes. Further characterization of the target glycolipids will have important implications for drug and vaccine development and will elucidate the poorly understood role of glycolipids in the immunology of infections."xsd:string |
| http://purl.uniprot.org/citations/23675550 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pntd.0002224"xsd:string |
| http://purl.uniprot.org/citations/23675550 | http://purl.uniprot.org/core/author | "Buxbaum L.U."xsd:string |
| http://purl.uniprot.org/citations/23675550 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23675550 | http://purl.uniprot.org/core/name | "PLoS Negl Trop Dis"xsd:string |
| http://purl.uniprot.org/citations/23675550 | http://purl.uniprot.org/core/pages | "e2224"xsd:string |
| http://purl.uniprot.org/citations/23675550 | http://purl.uniprot.org/core/title | "Leishmania mexicana infection induces IgG to parasite surface glycoinositol phospholipids that can induce IL-10 in mice and humans."xsd:string |
| http://purl.uniprot.org/citations/23675550 | http://purl.uniprot.org/core/volume | "7"xsd:string |
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