| http://purl.uniprot.org/citations/23708003 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23708003 | http://www.w3.org/2000/01/rdf-schema#comment | "Present practices for reprogramming somatic cells to induced pluripotent stem cells involve simultaneous introduction of reprogramming factors. Here we report that a sequential introduction protocol (Oct4-Klf4 first, then c-Myc and finally Sox2) outperforms the simultaneous one. Surprisingly, the sequential protocol activates an early epithelial-to-mesenchymal transition (EMT) as indicated by the upregulation of Slug and N-cadherin followed by a delayed mesenchymal-to-epithelial transition (MET). An early EMT induced by 1.5-day TGF-β treatment enhances reprogramming with the simultaneous protocol, whereas 12-day treatment blocks reprogramming. Consistent results were obtained when the TGF-β antagonist Repsox was applied in the sequential protocol. These results reveal a time-sensitive role of individual factors for optimal reprogramming and a sequential EMT-MET mechanism at the start of reprogramming. Our studies provide a rationale for further optimizing reprogramming, and introduce the concept of a sequential EMT-MET mechanism for cell fate decision that should be investigated further in other systems, both in vitro and in vivo."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncb2765"xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Chen C."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "He S."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Guo Y."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Pan G."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Pei D."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Sun H."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Qi J."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Zheng H."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Feng C."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/author | "Qin D."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/name | "Nat Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/pages | "829-838"xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/title | "Sequential introduction of reprogramming factors reveals a time-sensitive requirement for individual factors and a sequential EMT-MET mechanism for optimal reprogramming."xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/23708003 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23708003 |
| http://purl.uniprot.org/citations/23708003 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23708003 |