| http://purl.uniprot.org/citations/23736872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23736872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23736872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/23736872 | http://www.w3.org/2000/01/rdf-schema#comment | "Pseudomonas putida NCIMB 9866 utilizes p-cresol or 2,4-xylenol as a sole carbon and energy source. Enzymes catalyzing the oxidation of the para-methyl group of p-cresol have been studied in detail. However, those responsible for the oxidation of the para-methyl group in 2,4-xylenol catabolism are still not reported. In this study, real-time quantitative PCR analysis indicated pchC- and pchF-encoded p-cresol methylhydroxylase (PCMH) and pchA-encoded p-hydroxybenzaldehyde dehydrogenase (PHBDD) in p-cresol catabolism were also likely involved in the catabolism of 2,4-xylenol. Enzyme activity assays and intermediate identification indicated that the PCMH and PHBDD catalyzed the oxidations of 2,4-xylenol to 4-hydroxy-3-methylbenzaldehyde and 4-hydroxy-3-methylbenzaldehyde to 4-hydroxy-3-methylbenzoic acid, respectively. Furthermore, the PCMH-encoding gene pchF was found to be necessary for the catabolism of 2,4-xylenol, whereas the PHBDD-encoding gene pchA was not essential for the catabolism by gene knockout and complementation. Analyses of the maximum specific growth rate (μ m) and specific activity of the gene-knockout strain to different intermediates revealed the presence of other enzyme(s) with PHBDD activity in strain 9866. However, PHBDD played a major role in the catabolism of 2,4-xylenol in contrast to the other enzyme(s)."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-013-5001-z"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-013-5001-z"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-013-5001-z"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/author | "Zhou N.Y."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/author | "Zhou N.Y."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/author | "Chen Y.F."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/author | "Chen Y.F."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/author | "Chao H."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/author | "Chao H."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/name | "Appl. Microbiol. Biotechnol."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/name | "Appl. Microbiol. Biotechnol."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/pages | "1349-1356"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/pages | "1349-1356"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/title | "The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/title | "The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866."xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/volume | "98"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://purl.uniprot.org/core/volume | "98"xsd:string |
| http://purl.uniprot.org/citations/23736872 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23736872 |
| http://purl.uniprot.org/citations/23736872 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23736872 |