http://purl.uniprot.org/citations/23747316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/23747316 | http://www.w3.org/2000/01/rdf-schema#comment | "Messenger RNA binding proteins control post-transcriptional gene expression of targeted mRNAs. The RGG (arginine-glycine-glycine) domain of the AUF1/hnRNP-D mRNA binding protein is a regulatory region that is essential for protein function. The AUF1-RGG peptide, modeled on the RGG domain of AUF1, represses expression of the macrophage cytokine, VEGF. This report expands studies on the AUF1-RGG peptide and evaluates the role of post-translational modifications of the AUF1 protein. Results show that a minimal 31-amino acid AUF1-RGG peptide that lacks poly-glutamine and nuclear localization motifs retains suppressive activity on a VEGF-3'UTR reporter. Arginine residues in RGG motifs may be methylated with resulting changes in protein function. Mass spectroscopy analysis was performed on AUF1 expressed in RAW-264.7 cells. In resting cells, arginines in the first and second RGG motifs are monomethylated. Following activation with lipopolysaccharide, the arginines are dimethylated. To evaluate if the arginine residues are essential for AUF1-RGG activity, the methylatable arginines in the AUF1-3RGG peptide were mutated to lysine or alanine. The R→K and R→A mutants lack activity. We also demonstrate that PI3K/AKT inhibitors reduce VEGF gene expression. Although immunoscreening of AUF1 suggests that LPS and PI3K inhibitors alter the phosphorylation status of AUF1-p37, mass spectroscopy results show that the p37 AUF1 isoform is not phosphorylated with or without lipopolysaccharide stimulation. In summary, arginines in the RGG domain of AUF1 are methylated, and AUF1-RGG peptides may be novel reagents that reduce macrophage activation in inflammation."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.intimp.2013.05.014"xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/author | "Mierke D.F."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/author | "Deng B."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/author | "Fellows A."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/author | "Nichols R.C."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/author | "Robey R.B."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/name | "Int Immunopharmacol"xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/pages | "132-141"xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/title | "Peptides modeled on the RGG domain of AUF1/hnRNP-D regulate 3' UTR-dependent gene expression."xsd:string |
http://purl.uniprot.org/citations/23747316 | http://purl.uniprot.org/core/volume | "17"xsd:string |
http://purl.uniprot.org/citations/23747316 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23747316 |
http://purl.uniprot.org/citations/23747316 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23747316 |
http://purl.uniprot.org/uniprot/#_G5E8G0-mappedCitation-23747316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/#_G3X9W0-mappedCitation-23747316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/#_Q60668-mappedCitation-23747316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/#_Q3UXC8-mappedCitation-23747316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/Q3UXC8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/G3X9W0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/Q60668 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23747316 |
http://purl.uniprot.org/uniprot/G5E8G0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23747316 |