| http://purl.uniprot.org/citations/23756812 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23756812 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23756812 | http://www.w3.org/2000/01/rdf-schema#comment | "Glutaredoxins that contain a Cys-X-X-Cys active site motif are glutathione-dependent thiol-disulfide oxidoreductases. Vertebrate glutaredoxin 2 is characterized by two extra cysteines that form an intra-molecular disulfide bridge. Zebrafish glutaredoxin 2 contains four additional cysteines that are conserved within the infraclass of bony fish (teleosts). Here, we present a biochemical and biophysical characterization of zebrafish glutaredoxin 2, focusing on iron-sulfur-cluster coordination. The coordination of [2Fe2S](2+)-clusters in monomers of this protein was revealed by both absorption and Mössbauer spectroscopy as well as size exclusion chromatography. All other holo-glutaredoxins represent [FeS]-cluster bridged dimers using two molecules of non-covalently bound glutathione and the N-terminal active site cysteines as ligands. These cysteine residues were not required for [FeS]-cluster coordination in zebrafish glutaredoxin 2. A crystal structure of the teleost protein revealed high structural similarity to its human homologue. The two vertebrate-specific cysteines as well as two of the teleost-specific cysteines are positioned within a radius of 7Å near the C-terminus suggesting a potential role in [FeS]-cluster coordination. Indeed, mutated proteins lacking these teleost-specific cysteines lost the ability to bind the cofactor. Hence, the apparent mode of [FeS]-cluster coordination in zebrafish glutaredoxin 2 could be different from all yet described [FeS]-glutaredoxins."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2013.05.132"xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Berndt C."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Berndt C."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Brautigam L."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Brautigam L."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Bill E."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Bill E."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Johansson C."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Johansson C."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "McDonough M.A."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "McDonough M.A."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Holmgren A."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Holmgren A."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Kubsch B."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/author | "Kubsch B."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/pages | "491-496"xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/pages | "491-496"xsd:string |
| http://purl.uniprot.org/citations/23756812 | http://purl.uniprot.org/core/title | "An unusual mode of iron-sulfur-cluster coordination in a teleost glutaredoxin."xsd:string |