| http://purl.uniprot.org/citations/23791703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23791703 | http://www.w3.org/2000/01/rdf-schema#comment | "Several Cl(-) channels have been described in the native renal tubule, but their correspondence with ClC-K1 and ClC-K2 channels (orthologs of human ClC-Ka and ClC-Kb), which play a major role in transcellular Cl(-) absorption in the kidney, has yet to be established. This is partly because investigation of heterologous expression has involved rat or human ClC-K models, whereas characterization of the native renal tubule has been done in mice. Here, we investigate the electrophysiological properties of mouse ClC-K1 channels heterologously expressed in Xenopus laevis oocytes and in HEK293 cells with or without their accessory Barttin subunit. Current amplitudes and plasma membrane insertion of mouse ClC-K1 were enhanced by Barttin. External basic pH or elevated calcium stimulated currents followed the anion permeability sequence Cl(-)>Br(-)>NO3(-)>I(-). Single-channel recordings revealed a unit conductance of ~40pS. Channel activity in cell-attached patches increased with membrane depolarization (voltage for half-maximal activation: ~-65mV). Insertion of the V166E mutation, which introduces a glutamate in mouse ClC-K1, which is crucial for channel gating, reduced the unit conductance to ~20pS. This mutation shifted the depolarizing voltage for half-maximal channel activation to ~+25mV. The unit conductance and voltage dependence of wild-type and V166E ClC-K1 were not affected by Barttin. Owing to their strikingly similar properties, we propose that the ClC-K1/Barttin complex is the molecular substrate of a chloride channel previously detected in the mouse thick ascending limb (Paulais et al., J Membr. Biol, 1990, 113:253-260)."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamem.2013.06.012"xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "L'Hoste S."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Keck M."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Korbmacher C."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Lourdel S."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Teulon J."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Genete M."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Grand T."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Beck L."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Paulais M."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Pinelli L."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Diakov A."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/author | "Andrini O."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/pages | "2399-2409"xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/title | "Characterization of the mouse ClC-K1/Barttin chloride channel."xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://purl.uniprot.org/core/volume | "1828"xsd:string |
| http://purl.uniprot.org/citations/23791703 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23791703 |
| http://purl.uniprot.org/citations/23791703 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23791703 |
| http://purl.uniprot.org/uniprot/#_A2ADB4-mappedCitation-23791703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23791703 |
| http://purl.uniprot.org/uniprot/#_Q9WUB7-mappedCitation-23791703 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23791703 |
| http://purl.uniprot.org/uniprot/A2ADB4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/23791703 |