| http://purl.uniprot.org/citations/23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23795651 | http://www.w3.org/2000/01/rdf-schema#comment | "Chromosomal replication origins, where DNA replication is initiated, are determined in eukaryotic cells by specific binding of a six-subunit origin recognition complex (ORC). Many biochemical analyses have showed the detailed properties of the ORC-DNA interaction. However, because of the lack of in vitro analysis, the molecular architecture of the ORC-chromatin interaction is unclear. Recently, mainly from in vivo analyses, a role of chromatin in the ORC-origin interaction has been reported, including the existence of a specific pattern of nucleosome positioning around origins and of a specific interaction between chromatin-or core histones-and Orc1, a subunit of ORC. Therefore, to understand how ORC establishes its interaction with origin in vivo, it is essential to know the molecular mechanisms of the ORC-chromatin interaction. Here, we show that ORC purified from yeast binds more stably to origin-containing reconstituted chromatin than to naked DNA and forms a nucleosome-free region at origins. Molecular imaging using atomic force microscopy (AFM) shows that ORC associates with the adjacent nucleosomes and forms a larger complex. Moreover, stable binding of ORC to chromatin requires linker DNA. Thus, ORC establishes its interaction with origin by binding to both nucleosome-free origin DNA and neighboring nucleosomes."xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.org/dc/terms/identifier | "doi:10.1111/gtc.12073"xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/author | "Araki H."xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/author | "Yagura M."xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/author | "Hizume K."xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/pages | "764-779"xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/title | "Concerted interaction between origin recognition complex (ORC), nucleosomes and replication origin DNA ensures stable ORC-origin binding."xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/23795651 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23795651 |
| http://purl.uniprot.org/citations/23795651 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23795651 |
| http://purl.uniprot.org/uniprot/#_P0C5Y9-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P57053-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P58876-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P62805-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P62807-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P33778-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P84243-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P50874-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_O60814-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P06899-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P16104-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |
| http://purl.uniprot.org/uniprot/#_P04908-mappedCitation-23795651 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23795651 |