| http://purl.uniprot.org/citations/23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23801330 | http://www.w3.org/2000/01/rdf-schema#comment | "Calcium-dependent activator protein for secretion 1 (CAPS1) is a multidomain protein containing a Munc13 homology domain 1 (MHD1). Although CAPS1 and Munc13-1 play crucial roles in the priming stage of secretion, their functions are non-redundant. Similar to Munc13-1, CAPS1 binds to syntaxin-1, a key t-SNARE protein in neurosecretion. However, whether CAPS1 interacts with syntaxin-1 in a similar mode to Munc13-1 remains unclear. Here, using yeast two-hybrid assays followed by biochemical binding experiments, we show that the region in CAPS1 consisting of the C-terminal half of the MHD1 with the corresponding C-terminal region can bind to syntaxin-1. Importantly, the binding mode of CAPS1 to syntaxin-1 is distinct from that of Munc13-1; CAPS1 binds to the full-length of cytoplasmic syntaxin-1 with preference to its "open" conformation, whereas Munc13-1 binds to the first 80 N-terminal residues of syntaxin-1. Unexpectedly, the majority of the MHD1 of CAPS1 is dispensable, whereas the C-terminal 69 residues are crucial for the binding to syntaxin-1. Functionally, a C-terminal truncation of 69 or 134 residues in CAPS1 abolishes its ability to reconstitute secretion in permeabilized PC12 cells. Our results reveal a novel mode of binding between CAPS1 and syntaxin-1, which play a crucial role in neurosecretion. We suggest that the distinct binding modes between CAPS1 and Munc13-1 can account for their non-redundant functions in neurosecretion. We also propose that the preferential binding of CAPS1 to open syntaxin-1 can contribute to the stabilization of the open state of syntaxin-1 during its transition from "closed" state to the SNARE complex formation."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.494088"xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Park S."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Kim M.Y."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Sugita S."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Jung C.H."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Park S.'"xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Parsaud L."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/author | "Saw N.M."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/pages | "23050-23063"xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/title | "Calcium-dependent activator protein for secretion 1 (CAPS1) binds to syntaxin-1 in a distinct mode from Munc13-1."xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://purl.uniprot.org/core/volume | "288"xsd:string |
| http://purl.uniprot.org/citations/23801330 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23801330 |
| http://purl.uniprot.org/citations/23801330 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23801330 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DGY2-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YUA0-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |
| http://purl.uniprot.org/uniprot/#_A0A286YDH6-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DFZ1-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |
| http://purl.uniprot.org/uniprot/#_A6K086-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |
| http://purl.uniprot.org/uniprot/#_A6K087-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |
| http://purl.uniprot.org/uniprot/#_A0A286YE00-mappedCitation-23801330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23801330 |